| 大肠杆菌JM105的转化及快速鉴定 |
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| 引用本文： | 付桂莲,杜培革,项小丰,陈立娟,石秋,魏小平.大肠杆菌JM105的转化及快速鉴定[J].北华大学学报(自然科学版),1994(4). |
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| 作者姓名： | 付桂莲 杜培革 项小丰 陈立娟 石秋 魏小平 |
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| 作者单位： | 吉林医学院生化检验教研室
(付桂莲，杜培革，项小丰)，吉林市中心医院内科
(陈立娟，石秋)，吉林市中心医院药剂科(魏小平) |
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| 摘 要： | 用CaCL_2处理JM105空白的大肠杆菌,使之成为感受态细菌,然后将含白细胞介素-8(interleukin-8,IL-8)的cDNA的质粒PGEM-72f~(( ))转化到感受态细菌中去,涂布于含有氨苄青霉素的LB固体培养基中,培养过夜,挑取生长良好的单菌落,再接种到含氨苄青霉素的LB液体培养基中,培养过夜,用溶菌酶法裂解细菌细胞壁,用0.8％。琼脂糖凝胶电泳检测质粒,在紫外反射透射仪上观察结果。此方法简便,快速,不需特殊设备及试剂。
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| 关 键 词： | 质粒 转化 电泳 |
Transformation and Identification of E coli JM 105 |
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| Fu Gui-Lian et al.Transformation and Identification of E coli JM 105[J].Journal of Beihua University(Natural Science),1994(4). |
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| Authors: | Fu Gui-Lian |
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| Institution: | Dept. of Biochemistry |
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| Abstract: | Competent E coli JM 105 was prepared using Calcium chloride and transformed with plasmid PGEM -72f( )that contains IL -8. After transforming, bacteria were grown on agar medium (LB) containing ampicillin. when selecting for resistance to ampicillin, transformed cell was incubated in LB liguid medium and harvsted. Lysis of cell was achieved by lysozyme and 0.8% agarose gel electrophoresis was used for identificating the recombinat plasmid. This assay is easy and rapid. |
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| Keywords: | plasmid transformation electrophoresis |
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