| 中华鳖种群RAPD分析 |
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| 引用本文： | 肖亚梅,陈丽莉,陈合格,赵如榕.中华鳖种群RAPD分析[J].湖南师范大学自然科学学报,2005,28(3):72-75. |
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| 作者姓名： | 肖亚梅 陈丽莉 陈合格 赵如榕 |
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| 作者单位： | 湖南师范大学生命科学学院,中国,长沙,410081 |
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| 摘 要： | 选用14个随机引物对中华鳖基因组DNA进行了随机扩增DNA片段多态性（RAPD）分析，14个引物，在8个待检样品中共扩增出714条清晰的DNA片段，片段大小在200～3000bp之间，平均每一引物在每个样品中扩增了6．0条带，统计结果表明，中华鳖群体内平均相似率0．857，总平均带纹频率0．773，遗传变异率为0．227．研究表明，中华鳖为遗传共享度较高的群体。
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| 关 键 词： | 中华鳖 RAPD 遗传变异率 |
| 文章编号： | 1000-2537（2004）03-0072-04 |
| 收稿时间： | 2005-01-02 |
| 修稿时间： | 2005年1月2日 |
RAPD Analysis of Genomic DNA in the Pelodiscus Sinensis |
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| XIAO Ya-mei,CHEN Li-li,CHEN He-ge,ZHAO Ru-rong.RAPD Analysis of Genomic DNA in the Pelodiscus Sinensis[J].Journal of Natural Science of Hunan Normal University,2005,28(3):72-75. |
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| Authors: | XIAO Ya-mei CHEN Li-li CHEN He-ge ZHAO Ru-rong |
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| Institution: | College of Life Science, Hunan Normal University, Changsha 410081, China |
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| Abstract: | The genomic DNA of the Pelodiscus sinensis were ampilified with 14 random primers, then 714 bands were observed with 14 primers in 8 samples. The length of these DNA fragments were varied from 200 to 3 000 bp. On average, there existed about 6.0 bands being observed with single primer and each sample. The intra-population band sharing was 0. 857, average band frequency was 0. 773, and the genetic variability was 0. 227. |
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| Keywords: | Pelodiscus sinensis RAPD genetic variability |
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