Glutamine stimulates translation of uncoupling protein 2mRNA |
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Authors: | C Hurtaud C Gelly Z Chen C Lévi-Meyrueis F Bouillaud |
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Institution: | (1) BIOTRAM UPR9078, CNRS, Université Paris Descartes, Faculté de Médecine Necker Enfants Malades, 156 rue de Vaugirard, 75730 Paris, France |
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Abstract: | Uncoupling protein 2 (UCP2) belongs to a family of transporters/exchangers of the mitochondrial inner membrane. Using cell
lines representing natural sites of UCP2 expression (macrophages, colonocytes, pancreatic beta cells), we show that UCP2 expression
is stimulated by glutamine at physiological concentrations. This control is exerted at the translational level. We demonstrate
that the upstream open reading frame (ORF1) in the 5’ untranslated region (5’UTR) of the UCP2 mRNA is required for this stimulation
to take place. Cloning of the 5’ UTR of the UCP2 mRNA in front of a GFP cDNA resulted in a reporter gene with which GFP expression
could be induced by glutamine. An effect of glutamine on translation of a given mRNA has not been identified before, and this
is the first evidence for a link between UCP2 and glutamine, an amino acid oxidized by immune cells or intestinal epithelium
and playing a role in the control of insulin secretion.
Received 26 January 2007; received after revision 16 April 2007; accepted 8 May 2007
C. Hurtaud, C. Gelly: These authors contributed equally to this work. |
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Keywords: | Glutamine mitochondria UCP2 5’ untranslated region translation green fluorescent protein |
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