黄芩愈伤组织培养及细胞形态学观察

采用不同的植物激素、碳源、水解乳蛋白(LH)和水解酪蛋白(CH)、光暗条件对黄芩愈伤组织进行培养,通过石蜡切片观察愈伤组织细胞形态。结果表明:0.5mg/LNAA和2.0mg/LKt组合时愈伤组织生长量最多;1.0mg/L2,4-D和0.5mg/LKt组合时,黄芩甙含量最高;蔗糖为2%,葡萄糖为3%或4%时,都不利于黄芩甙的积累;LH、CH、黑暗条件能促进愈伤组织的生长而不利于黄芩甙的积累;光照能显著提高黄芩甙的含量;胚性愈伤组织占优势,已出现明显的微管组织的分化。

Callus culturing and cytomorphology observation of Scutellaria Baicalensis

Callus of Scutellaria Baicalensis was treated by plant hormone,carbon source,lactalbumin hydrolysate(LH),casein hydrolysate(CH),illumination and dark.Cytomorphology was observed by paraffin embedded section.The results show that 0.5mg/L NAA and 2.0mg/L Kt effectively increased yield of callus;the highest baicalin content was achieved at 1.0mg/L 2,4-D and 0.5mg/L Kt;sucrose at 2% and glucose at 3% or 4% did not favor accumulation of baicalin;LH,CH and dark increased yield of callus but had depressing effects on accumulation of baicalin;illumination could raise baicalin content;embryogenic callus was dominent;the obvious differentiation of microtubule tissue was found in callus.