| 海岛棉ERF族转录因子EREB6基因克隆特征分析 |
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| 引用本文： | 刘坤,单国芳,李付广,刘传亮,张朝军,张雪妍,武芝侠. 海岛棉ERF族转录因子EREB6基因克隆特征分析[J]. 河南师范大学学报(自然科学版), 2011, 39(2): 106-110 |
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| 作者姓名： | 刘坤 单国芳 李付广 刘传亮 张朝军 张雪妍 武芝侠 |
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| 作者单位： | 中国农业科学院棉花研究所,农业部棉花遗传改良重点开放实验室,河南安阳455004 |
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| 基金项目： | 国家863项目(2006AA100105) |
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| 摘 要： | 以电子克隆,同源扩增和RACE技术相结合的方法从海岛棉中克隆出了一条新基因,命名为EREB6,它包含一个669 bp长的开放阅读框,预期编码222个氨基酸长的蛋白质.序列分析发现预期蛋白含有一段核定位信号序列,GFP融合蛋白瞬时表达实验证明该蛋白定位于细胞核；含有一个保守的ERF域,属于ERF族；同源分析表明该转录因子...
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| 关 键 词： | 转录因子 ERF 海岛棉 EREB6 |
Cloning and Characteristcs Analysis of EREB6, a Novel Transcription Factor Gene of ERF Family from Gossypium barbadense L |
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| LIU Kun,SHAN Guo-fang,LI Fu-guang,LIU Chuan-liang,ZHANG Chao-jun,ZHANG Xue-yan,WU Zhi-xia. Cloning and Characteristcs Analysis of EREB6, a Novel Transcription Factor Gene of ERF Family from Gossypium barbadense L[J]. Journal of Henan Normal University(Natural Science), 2011, 39(2): 106-110 |
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| Authors: | LIU Kun SHAN Guo-fang LI Fu-guang LIU Chuan-liang ZHANG Chao-jun ZHANG Xue-yan WU Zhi-xia |
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| Affiliation: | (Cotton Research Institute,Chinese Academy of Agriculture Sciences;Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Aanyang 455004,China) |
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| Abstract: | In this study,a novel gene,designated EREB6 including an open reading frame with 669 bp,and coding a putative protein with 222 aa,is cloned from sealand cotton using in silico metods combined with PCR and RACE techniques.Sequence analysis show that it shares an ERF conserved domain with AtERF13,Pti4 and TiERF1,and it belongs to B3a group of ERF subfamily,and also indicates that the induced protein contains a nucleus localization signals,which is ditermined by subcellular localization experiment.The memebers of B3 group have the most homologous sequence with EREB6,which suggests that EREB6 maybe possess the similar function with them. |
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