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雪莲果染色体数目及核型分析
引用本文:李建军,杨影丽,范念斯,李景原,周延清. 雪莲果染色体数目及核型分析[J]. 河南师范大学学报(自然科学版), 2011, 39(2): 131-134
作者姓名:李建军  杨影丽  范念斯  李景原  周延清
作者单位:河南师范大学 生命科学学院,河南新乡,453007
基金项目:国家“十一五”科技支撑项目(2006BRI06R12-06);新乡市科技发展计划项目(09N028)
摘    要:雪莲果为菊科多年生双子叶草本植物,容易产生变异形成新的种系,为了确定我国引种雪莲果的染色体数目,采用0.002 M的8-羟基喹啉溶液预处理雪莲果根尖3~4 h,经卡诺氏液固定、酸解去壁、卡宝品红染色等步骤,对雪莲果进行了染色体数目的确定及体细胞染色体核型分析,结果表明雪莲果的染色体数目为2n=52=30m+22sm,核...

关 键 词:雪莲果  染色体  核型  核型分析  核型模式图

Chromosome Number and Karyotype Analysis of Yacon
LI Jian-jun,YANG Ying-li,FAN Nian-si,Li Jing-yuan,Zhou Yan-qing. Chromosome Number and Karyotype Analysis of Yacon[J]. Journal of Henan Normal University(Natural Science), 2011, 39(2): 131-134
Authors:LI Jian-jun  YANG Ying-li  FAN Nian-si  Li Jing-yuan  Zhou Yan-qing
Affiliation:(College of Life sciences,Henan Normal University,Xinxiang 453007,China)
Abstract:Yacon(Smallanthus sonchifolius) is the Asteraceae perennial dicotyledonous herbs,easy to produce the variation form new species,in order to determine our introduction Yacon number of chromosomes,pretreat yacon Root by 8-hydroxyquinoline solution(0.002 M)for 3~4 h,then fix it by Carnoy's solution,degrade cell wall by acid solution,stain it by Carbol fuchsin,etc.The research ascertains chromosome number of yacon and analysis on chromosme karyotype of yacon somatic cell.The analysis indicates that the chromosome number of yacon is 2n=52=30m+2sm,and its nuclear type is classified to "2B" of Stebbins classification.
Keywords:Yacon  chromosome  karyotype  karyotype analysis  idiogram
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