大孔吸附树脂对柚皮甙的吸附分离

通过比较5种吸附树脂对柚皮甙的吸附能力,选择了对柚皮甙吸附能力较强,且容易洗脱的吸附树脂X-5,实现了柚皮甙的吸附分离.研究了提取液浓度、pH值、流速等因素对柚皮甙在该树脂上吸附的影响,同时考察了解吸时洗脱剂浓度、pH值、流速等因素对柚皮甙在吸附树脂上解吸的影响.研究结果表明:柚皮甙在X-5大孔吸附树脂的吸附行为可以用Langmuir方程进行描述;当提取液质量浓度为2.7 g/L时,树脂具有最大饱和吸附容量32.6 mg/g;pH值对其吸附影响较弱;当每小时通过的溶剂体积为树脂体积的2倍时,动态吸附时动态饱和吸附容量为23.8 mg/g;pH约为10、体积分数为60%的乙醇水溶液为最佳洗脱剂;当每小时通过的洗脱剂体积为树脂体积的1～2倍时,洗脱率可达85%以上.

Adsorption and Separation of Naringin with Macroporous Resin

X-5 resin, which has higher adsorption capacity and easier desorption, was selected from five kinds of macroporous resins. Effects of the concentration, pH value and flow velocity of naringin on the adsorption were studied. Meanwhile, these factors on the desorption were also (evaluated.) The results show that the adsorption isotherm can be described with the Langmuir equation. The static maximum adsorption capacity for naringin is 32.6 mg/g when the concentration is 2.7 g/L.The dynamic adsorption capacity for naringin is 23.8 mg/g when the flow velocity is 2 times of resins volume per hour. The optimal eluting solvent is 60%(volume fraction) ethanol-water solution with pH value of 10. The desorption rate overpasses 85% when the flow velocity is 12 times of resins volume per hour.