| 离子液体双水相中茜素红S与牛血清白蛋白作用研究 |
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| 引用本文： | 朱陈银,邓凡政,高贺军.离子液体双水相中茜素红S与牛血清白蛋白作用研究[J].淮北煤炭师范学院学报(自然科学版),2009,30(3):29-31. |
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| 作者姓名： | 朱陈银 邓凡政 高贺军 |
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| 作者单位： | 淮北煤炭师范学院化学与材料科学学院,安徽,淮北,235000 |
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| 基金项目： | 安徽省教育厅自然科学研究重点项目 |
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| 摘 要： | 在离子液体四氟硼酸1-丁基-3-甲基咪唑(fBrnim]BF4)和NaH2PO4形成的双水相体系中,研究茜素红S(ARS)与牛血清白蛋白(BSA)生成复合物光谱行为和溶液酸度,盐浓度,离子液体用量,共存物质等对体系复合物测定影响.离子液体用量1.5 mL,磷酸二氢钠2.0 g,ARS 1.0 mL,蛋白质溶液1.0 mL,pH 6时ARS与BSA复合物吸光度较大.离子液相中复合物最大吸收波长位于531 nm,比单纯ARS红移109 nm;摩尔吸光系数(ε)为2.32×104L·mol-1·cm-1.用加入不同类型表面活性剂方法,初步探讨了ARS与BSA之间的作用机理.使用后的离子液体经过简单处理可以重复使用.
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| 关 键 词： | 离子液体 茜素红S 牛血清白蛋白 |
Study on the Interaction Between Alizarin Red S and Bovine Serum Albumin in Ionic Liquid Aqueous Two-phase System |
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| ZHU Chen-yin,DENG Fan-zheng,GAO He-jun.Study on the Interaction Between Alizarin Red S and Bovine Serum Albumin in Ionic Liquid Aqueous Two-phase System[J].Journal of Huaibei Coal Industry Teachers College(Natural Science edition),2009,30(3):29-31. |
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| Authors: | ZHU Chen-yin DENG Fan-zheng GAO He-jun |
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| Institution: | ZHU Chen-yin,DENG Fan-zheng,GAO He-jun (School of Chemistry and Material Science,Huaibei Coal Industry Teachers College,235000,Huaibei,Anhui,China) |
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| Abstract: | In ionic liquid Bmim] BF4 (1-butyl-3-methylimidazoliumtetrafluoroborate) and NaH2PO4 aqueous two-phase system (ATPS),the spectroscopic behaviour of the compound of alizarin red S (ARS) and bovine serum albumin (BSA) was investigated.Effects of different acidity,quantities of ionic liquid and salt and coexistent matter on the determination of system were discussed.In the ionic liquid aqueous two-phase system of a pH 6,1.0 mL ARS,1.5 mL Bmim] BF4 and 2.0 g NaH2PO4,the compound formed by ARS and BSA had high... |
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| Keywords: | ionic liquid alizarin red S bovine serum albumin |
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