| 家蚕血液肽聚糖识别蛋白的鉴定和生物信息学分析 |
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| 引用本文： | 李季生,毛晓霞,贾漫丽,李娜,高玉军,杨贵明.家蚕血液肽聚糖识别蛋白的鉴定和生物信息学分析[J].河南师范大学学报(自然科学版),2015(2):126-131. |
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| 作者姓名： | 李季生 毛晓霞 贾漫丽 李娜 高玉军 杨贵明 |
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| 作者单位： | 承德医学院河北省高校特产蚕桑技术研发中心;承德医学院蚕业研究所;承德医学院中药研究所 |
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| 基金项目： | 河北省教育厅高校科学技术研究计划(Q2012026) |
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| 摘 要： | 采用双向电泳和质谱技术分析、鉴定了家蚕血液中存在的肽聚糖识别蛋白(PGRP).为了进一步研究该蛋白的结构和功能,采用了生物信息学方法对其理化性质、疏水性/亲水性、信号肽、二级结构、三级结构、亚细胞定位和GO功能注释等进行了分析.结果发现,肽聚糖识别蛋白是一种分泌类蛋白,可能具有信号肽.其主要分子功能为肽聚糖受体,在生物学进程中发挥的主要作用为肽聚糖代谢和先天性免疫应答.
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| 关 键 词： | 家蚕 血液 肽聚糖识别蛋白 双向电泳 质谱 生物信息学 |
Identification and Bioinformatics Analysis on Peptidoglycan Recognition Protein of Silkworm Haemolymph |
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| LI Jisheng;XIA Aihua;MAO Xiaoxia;JIA Manli;LI Na;GAO Yujun;YANG Guiming.Identification and Bioinformatics Analysis on Peptidoglycan Recognition Protein of Silkworm Haemolymph[J].Journal of Henan Normal University(Natural Science),2015(2):126-131. |
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| Authors: | LI Jisheng;XIA Aihua;MAO Xiaoxia;JIA Manli;LI Na;GAO Yujun;YANG Guiming |
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| Institution: | LI Jisheng;XIA Aihua;MAO Xiaoxia;JIA Manli;LI Na;GAO Yujun;YANG Guiming;Hebei Universities R&D Centre for Sericulture and Specialty Enabling Technologies,Chengde Medical University;Institute of Sericulture,Chengde Medical University;Institute of Chinese Materia Medica,Chengde Medical University; |
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| Abstract: | Utilizing two-dimensional electrophoresis and mass spectrometry,peptidoglycan recognition protein(PGRP)was identified in the present study.In order to further analysis the structure and function of the protein,we empolyed bioinformatics methods to study its physical and chemical properties,hydrophobic/hydrophilic,signal peptide,secondary structure and tertiary structure,subcellular localization and functional annotations.Results showed that as a kind of secreted protein PGRP might have signal peptide and its molecular function was peptidoglycan receptor.In biological process ontology,it may play important role in peptidoglycan catabolic and innate immune response. |
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| Keywords: | silkworm haemolymph peptidoglycan recognition protein two-dimensional electrophoresis mass spectrometry bioinformactics |
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