| 响应面法优化小刺猴头菌寡糖的纯化工艺研究 |
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| 引用本文： | 孙文怡,刘金阳,刘瑜,宋慧. 响应面法优化小刺猴头菌寡糖的纯化工艺研究[J]. 长春师范学院学报, 2011, 0(10): 59-62 |
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| 作者姓名： | 孙文怡 刘金阳 刘瑜 宋慧 |
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| 作者单位： | 吉林师范大学生命科学学院;农业部规划设计研究院;吉林农业大学生命科学学院; |
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| 摘 要： | 采用盐析法对小刺猴头菌寡糖进行纯化,在pH值、硫酸铵饱和度和溶液浓度3个单因素试验的基础上,根据中心组合(Box-Benhnken)试验设计原理,以粗多糖中的蛋白去除率为指标响应值作响应面和等高线,分析各个因素的显著性和交互作用,得出利用硫酸铵盐析法去除寡糖中杂蛋白的工艺条件为:pH值为5.91,硫酸铵饱和度为57%,溶液浓度为4%。
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| 关 键 词： | 响应面分析 小刺猴头菌寡糖 硫酸铵 |
On the Optimization of Purification Technique of Oligosaccharide of H.caput-medusae(Bull.Fr.) Pers.using Response Surface Method |
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| SUN Wen-yi,LIU Jin-yang,LIU Yu,SONG Hui. On the Optimization of Purification Technique of Oligosaccharide of H.caput-medusae(Bull.Fr.) Pers.using Response Surface Method[J]. Journal of Changchun Teachers College, 2011, 0(10): 59-62 |
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| Authors: | SUN Wen-yi LIU Jin-yang LIU Yu SONG Hui |
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| Affiliation: | SUN Wen-yi1,LIU Jin-yang1,LIU Yu2,SONG Hui3*(1.College of Life Science,Jilin Normal University,Siping 136000,China,2.Chinese Academy of Agricultural Engineering,Beijing 100125,3.College of Life Sciences,Jilin Agricultural University,Changchun 130118,China) |
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| Abstract: | The experiment was conducted using salting-out method to purify the oligosaccharide of H.caput-medusae(Bull.Fr.) Pers..The pH,the saturation of ammonium sulfate and the solution concentration were investigated using single-factor experiment.Then the purification conditions of oligosaccharide of H.caput-medusae(Bull.Fr.) Pers.on the rationale of Box-Benhnken design of Response Surface Methodology experiment were optimized,and the response surface and contour were drawn out with the removal rate of protein in... |
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| Keywords: | response surface methodology oligosaccharide of H.caputmedusae(Bull.Fr.) Pers. ammonium sulfate |
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