A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue |
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| Authors: | Zhen Zhang JingFu Liu Yan Yao GuiBin Jiang |
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| Institution: | (1) Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China;(2) Department of Isotope, China Institute of Atomic Energy, Beijing, 102413, China; |
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| Abstract: | A novel dual-label time-resolved fluoroimmunoassay method was developed for the simultaneous determination of chloramphenicol
(CAP) and ractopamine (RAC) residues in 18 swine tissue samples, using anti-CAP and anti-RAC monoclonal antibodies labeled
with europium (Eu3+) and samarium (Sm3+), respectively. The detection limits for CAP and RAC were 0.06 and 0.25 ng/g. The recovery from swine muscle samples was
102%–121% for CAP at spiking levels of 0.1–5 ng/g, and 69.8%–85.8% for RAC at spiking levels of 1–10 ng/g. The results obtained
from the swine tissue samples using this method showed good agreement with those obtained using ELISA and GC-MS, with correlation
coefficients (R) between 0.92–0.98. |
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| Keywords: | dual-label time-resolved fluoroimmunoassay chloramphenicol ractopamine residue swine tissue |
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