| 细叶百日草变异植株的组织培养及无性系建立 |
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| 引用本文： | 刘立岩,王关林.细叶百日草变异植株的组织培养及无性系建立[J].辽宁师范大学学报(自然科学版),2009,32(2):239-242. |
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| 作者姓名： | 刘立岩 王关林 |
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| 作者单位： | 1. 铁岭师范高等专科学校,辽宁,铁岭,112001
2. 辽宁师范大学,生命科学院,辽宁,大连,116029 |
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| 摘 要： | 以变异细叶百日草嫩茎为材料，进行了愈伤组织的诱导、分化、试管苗生根、移栽的研究．结果表明：MS＋BA1．0＋2，4-D1．2为嫩茎愈伤组织诱导培养和继代培养的理想培养基；MS＋Ag（NO3）2 2．0＋BA0．4是愈伤组织和不定芽分化培养的理想培养基；1／3MS＋IAA0．5是生根培养的理想培养基；以炉灰渣为基质移栽的成活率为94％，扦插的成活率为89％；定植成活的试管苗保持了后期生长很旺盛变异性状，出现了根系增加1倍左右的特点．
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| 关 键 词： | 细叶百日草 组织培养 无性系 |
Tissue cultivation and establishment of asexual line of Zinnia linearis |
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| LIU Li-yan,WANG Guan-lin.Tissue cultivation and establishment of asexual line of Zinnia linearis[J].Journal of Liaoning Normal University(Natural Science Edition),2009,32(2):239-242. |
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| Authors: | LIU Li-yan WANG Guan-lin |
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| Institution: | LIU Li-yan , WANG Guan-lin (1. Tieling Teachers College, Tieling 112001, China; 2. College of Life Sciences, Liaoning Normal University, Dalian 116029, China) |
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| Abstract: | The study on callus induction, differentiation, rooting and transplantation of tube seedling was taken by using young stem as material. The results indicated that the ideal medium for induction and differentiation of callusare were MS＋ BA1 mg/L＋2,4-D1.2 mg/L＋2,4-D0.6 mg/L and MS＋ BA0.5 mg/L＋NAA0. 1 mg/L, respectively. The effective medium for rooting was 1/3MS＋IAA 0.5 mg/L＋IAA0.2 mg/L. The surviving rate of transplantation and cutting was 94% and 89% respectively by using cinder as matrix. The seedling keeps the exuberant growth and the number of roots was doubled. |
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| Keywords: | Zinnia linearis cultivation of tissue clone |
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