| 葡萄糖氧化酶在管状空心SiO2载体上的固定化 |
| |
| 引用本文： | 蒋利伟,袁其朋,肖清贵.葡萄糖氧化酶在管状空心SiO2载体上的固定化[J].北京化工大学学报(自然科学版),2007,34(4):428-431. |
| |
| 作者姓名： | 蒋利伟 袁其朋 肖清贵 |
| |
| 作者单位： | 北京化工大学 1生命科学与技术学院； 2教育部超重力工程研究中心， 北京 100029 |
| |
| 摘 要： | 以针状CaCO3为无机模板，制作成新型固定化材料管状空心SiO2载体，用来固定葡萄糖氧化酶(GOD).研究了pH值，温度和载体与GOD的配比等对固定化酶活的影响。所得的最佳工艺条件为：最佳载体和游离酶配比为0.6g/mL;固定化酶的最适pH值为5.2;最佳反应温度为32℃.和游离酶相比无论操作性还是稳定性都有提高。
|
| 关 键 词： | 固定化酶 葡萄糖氧化酶 管状空心SiO2 针状碳酸钙 固定化酶 葡萄糖氧化酶 管状空心SiO2 针状碳酸钙 |
| 修稿时间： | 2007-02-05 |
Hollow silica nanotubes for immobilization of glucose oxidase enzyme |
| |
| JIANG LiWei,YUAN QiPeng,XIAO QingGui.Hollow silica nanotubes for immobilization of glucose oxidase enzyme[J].Journal of Beijing University of Chemical Technology,2007,34(4):428-431. |
| |
| Authors: | JIANG LiWei YUAN QiPeng XIAO QingGui |
| |
| Institution: | 1College of Life Science and Technology; 2Research Center of the Ministry of Education for High Gravity Engineering and Technology, Beijing University of Chemical Technology, Beijing 100029, China |
| |
| Abstract: | Porous hollow silica nanotubes (PHSNTs) have been synthesized via a sol-gel route using nano-sized needle-like CaCO3 as the inorganic template and employed as a support for immobilization of Glucose Oxidase enzyme (GOD) biocatalyst. The effect of varying factors such as phosphate buffer pH, immobilization temperature and ratio of carrier to free GOD (g/mL) on the activity of immobilized GOD are discussed. The optimum conditions were found to be as follows: ratio of carrier to free GOD (g/mL) 0.6g/mL, phosphate buffer pH 5.2, immobilization temperature 28℃. The results show that under optimized conditions the efficacy and stability of GOD are improved relative to the free enzyme. |
| |
| Keywords: | enzyme immobilization glucose oxidase enzyme(GOD) hollow silica nanotubes needle-like CaCO3 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《北京化工大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《北京化工大学学报(自然科学版)》下载免费的PDF全文 |
