| Programmed cell death during terminal bud senescence in a sympodial branching tree, Eucommia ulmoides |
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| 作者姓名： | XU Wenjie Kalima-N'Koma MWANGE CUI Keming |
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| 作者单位： | College of Life Sciences, Peking University, Beijing 100871, China,College of Life Sciences, Peking University, Beijing 100871, China,College of Life Sciences, Peking University, Beijing 100871, China |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家自然科学基金 |
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| 摘 要： | Eucommia ulmoides Oliv. is a typical sympodial branching tree. The apical bud of the branch ages and dies every year, replaced by the nearby axillary bud in the second year. Structural assays and a series of biochemical analyses were performed to analyze the senescence mechanism in the apical bud. It was revealed that most cells of the apical bud underwent the programmed cell death (PCD) during the senescence: the chromosomes were congregated and the nuclear contents were condensed, as shown by 4′,6-diamidino-2-phenylindole (DAPI) fluorescence. DNA fragmentation was detected during senescence using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labeling (TUNEL) method, coincident with the appearance of a DNA ladder. Moreover, a 20 kD DNase related to fragmentation was found. PCD was initiated first in the young leaves, leaf primordia and peripheral zone cells, then in the central mother cells and initial layer cells in the apical meristem. The terminal buds remain in vegetative growth during senescence, in contrast to buds of many annual plants.
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| 关 键 词： | DNA fragmentation DNase Eucommia ulmoides Oliv. programmed cell death terminal bud senescence s |
Programmed cell death during terminal bud senescence in a sympodial branching tree, Eucommia ulmoides |
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| XU Wenjie,Kalima-N''Koma MWANGE,CUI Keming.Programmed cell death during terminal bud senescence in a sympodial branching tree, Eucommia ulmoides[J].Progress in Natural Science,2004,14(8):694-699. |
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| Authors: | XU Wenjie Kalima-N'Koma MWANGE CUI Keming |
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| Institution: | College of Life Sciences, Peking University, Beijing 100871, China |
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| Abstract: | Eucommia ulmoides Oliv. is a typical sympodial branching tree. The apical bud of the branch ages and dies every year, replaced by the nearby axillary bud in the second year. Structural assays and a series of biochemical analyses were performed to analyze the senescence mechanism in the apical bud. It was revealed that most cells of the apical bud underwent the programmed cell death (PCD) during the senescence: the chromosomes were congregated and the nuclear contents were condensed, as shown by 4′,6-diamidino-2-phenylindole (DAPI) fluorescence. DNA fragmentation was detected during senescence using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labeling (TUNEL) method, coincident with the appearance of a DNA ladder. Moreover, a 20 kD DNase related to fragmentation was found. PCD was initiated first in the young leaves, leaf primordia and peripheral zone cells, then in the central mother cells and initial layer cells in the apical meristem. The terminal buds remain in vegetative growth during senescence, in contrast to buds of many annual plants. |
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| Keywords: | DNA fragmentation DNase Eucommia ulmoides Oliv programmed cell death terminal bud senescence sympodium |
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