低能碳离子对甜菊生长和叶绿体发育的影响

研究了能量为１００ｋｅＶ，剂量为１０＾１５／ｃｍ＾２的碳离子对甜菊种子萌发率、幼苗生长发育及叶绿体结构的影响，证明被注入种子出现萌发迟缓、生长速度减慢、植株型变矮和生物量减少等生物学性状变化，幼叶细胞的叶绿体发育分化减慢，基质类囊体形成滞后，基粒数及基粒类囊体片层减少，部分幼叶细胞叶绿体膜破损、基质片层断裂直至叶绿体解体，表明该能量剂量的碳离子注入将影响种子的生长发育，其原因之一是叶绿全发育迟缓和

Effects of Low-energy Carbon Ion Implantation on the Growth and Development of Chloroplast of Stevia rebaudiana

This paper reports the effects of carbon ion implantation at 100 keV and 10 15 /cm 2 on the seed germination rate, development, growth, and chloroplast structure of Stevia rebaudiana seedling. The result showed the following changes of the biological characters of the ion implanted seeds in:(1) the retardation of the seed germination, the deceleration of the growth speed, the stunt of plant height, and the decrease of biomass;(2)the deceleration of differentiation and growth of the chloroplast in young leaf cells, the retardation in forming thylakoid, the decrease of the grana numbers and thylakoid lamella; and (3) the damage of the chloroplast membrane, the cleavage of stroma lamella and/or the decomposition of chloroplast(ca.5%).The experimental result proved that the carbon ion implantation at the above energy and dose surely influenced the development and growth of S. rebaudiana seed because of the lesion and development retardation of chloroplast. The study suggested a reference to the induced breeding of S. rebaudiana by low energy carbon ion implantation.