| 紫外诱变原生质体提高脂肪酶活力 |
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| 引用本文： | 晋青波,张庆庆,汤斌,危勤涛. 紫外诱变原生质体提高脂肪酶活力[J]. 安徽工程科技学院学报：自然科学版, 2008, 23(1): 7-10 |
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| 作者姓名： | 晋青波 张庆庆 汤斌 危勤涛 |
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| 作者单位： | 安徽工程科技学院,微生物发酵安徽省工程技术研究中心,安徽,芜湖,241000 |
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| 基金项目： | 安徽省教育厅自然科学基金 |
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| 摘 要： | 以粗壮假丝酵母CJ-1为出发菌株,对其进行原生质体紫外诱变选育.筛选得到10株脂肪酶活力比出发菌株高的突变株,其中菌株CJ-1-09的酶活达35.78 U/mL,比CJ-1提高了4.58倍.突变株CJ-1-09经10次传代,其脂肪酶活性保持稳定.
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| 关 键 词： | 粗壮假丝酵母 脂肪酶 原生质体 紫外诱变 |
| 文章编号： | 1672-2477(2008)01-0007-04 |
| 修稿时间： | 2007-11-05 |
Breeding of higher lipase-activity mutants by protoplast mutagenesis of ultraviolet irriation |
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| JIN Qing-bo,ZHANG Qing-qing,TANG-bin,WEI Qin-tao. Breeding of higher lipase-activity mutants by protoplast mutagenesis of ultraviolet irriation[J]. Journal of Anhui University of Technology and Science, 2008, 23(1): 7-10 |
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| Authors: | JIN Qing-bo ZHANG Qing-qing TANG-bin WEI Qin-tao |
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| Abstract: | Candida valida CJ-1 was induced by protoplast mutagenesis of ultraviolet irriation and ten mutants were obtained.The best strain was CJ-1-09,whose lipase activity was 35.78 U/mL,increased by 4.58 times in comparison with Candida valida CJ-1.Lipase activity keeps stable after 10 generations of reproduction,which displays perfect genetic stability. |
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| Keywords: | Candida valida Lipase Protoplast Ultraviolet mutagenesis |
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