| 用聚乙二醇分离人血清免疫球蛋白 |
| |
| 引用本文： | 林少琴,吴旭初,余萍,朱苏闽,余宝笙.用聚乙二醇分离人血清免疫球蛋白[J].福建师范大学学报(自然科学版),1987(1). |
| |
| 作者姓名： | 林少琴 吴旭初 余萍 朱苏闽 余宝笙 |
| |
| 作者单位： | 福建师范大学高分子研究所，福建师范大学高分子研究所，福建师范大学高分子研究所，福建师范大学高分子研究所，福建师范大学高分子研究所 |
| |
| 摘 要： | 作者用聚乙二醇(PEG)分离了人血清免疫球蛋白(IgG),实验表明该方法比经典的硫酸铵沉淀法具有更高的特异性和回收率。探讨了PEG的分子量、浓度及体系的pH、离子强度对分离人IgG的影响。表明在pH7.8的0.025M PBS体系中采用16％的PEG(M.W.1000)效果最佳。
|
Experiment on Isolating-Human IgG with Polyethylen eglycol |
| |
| Lin Shao-qin Wu Xu-chu Yu Ping Zhu Su-min Yu Bao-shen.Experiment on Isolating-Human IgG with Polyethylen eglycol[J].Journal of Fujian Teachers University(Natural Science),1987(1). |
| |
| Authors: | Lin Shao-qin Wu Xu-chu Yu Ping Zhu Su-min Yu Bao-shen |
| |
| Institution: | Institute of Polymer Science |
| |
| Abstract: | This parer reports a method using polyethyleneglycol (PEG) to isolate human IgG. The method has much more specific activity and recovery as compared to clsssical method of ammonium sulfate precipitation. Affect on pH and ionic strengh of the system and molecular weight and concentration of PEG to isolating human IgG was studied. Using 16%PEG (M.W.1000)could achieve the best result at 0.025M PBS, pHT. 8. |
| |
| Keywords: | Human IgG Isolating Polyethyleneglycol |
| 本文献已被 CNKI 等数据库收录！ |
