Exchange of tritium from randomly tritiated taurocholate by microbial bile salt oxidoreductases |
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Authors: | I A Macdonald |
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Institution: | (1) Department of Medicine, Dalhousie University Halifax, B3H 1W2 Nova Scotia, Canada |
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Abstract: | Summary Tritium distribution on randomly labelled taurocholate (TC) was estimated at 28%, 4%, 1% and <0.5% on the hydrogens opposite the 3 -, 7 - and 12 -OH groups and taurine moiety respectively. Anomalously,C. perfringens 3 -hydroxysteroid dehydrogenase (3 -HSDH) catalyzed tritium loss of 36% on formation of 7 -, 12 -dihydroxy-3-keto-5 -cholanoate, implying additional losses of tritium at other sites by this enzyme.This work is supported by the National Cancer Institute. |
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