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| 高产木糖醇酵母菌的筛选鉴定及两级法发酵特性 | |
| 引用本文： | 张金明,耿安利,姚传义,卢英华,李清彪.高产木糖醇酵母菌的筛选鉴定及两级法发酵特性[J].厦门大学学报(自然科学版),2011,50(3):624-630. |
| 作者姓名： | 张金明 耿安利 姚传义 卢英华 李清彪 |
| 作者单位： | 1. 厦门大学化学化工学院,福建厦门,361005 2. 义安理工学院生命与化学技术学院,新加坡,599489 3. 厦门大学化学化工学院,福建厦门361005;厦门大学化学生物学福建省重点实验室,福建厦门361005 |
| 摘 要： | 从300多种土壤样品中进行筛选,最终得到10株可耐受300 g/L木糖的酵母菌株,且其木糖醇转化率超过64.0%.其中酵母菌株SB18在150 g/L木糖下的木糖醇转化率为68.5%,为相同条件下转化率最大的菌株,并经18SrDNA测序鉴定为热带假丝酵母.酶活测定结果显示SB18木糖还原酶主要依赖辅因子还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH),其酶活性远大于木糖醇脱氢酶活性.对SB18进行两级法发酵条件研究,确定补充氮源为尿素和酵母提取物,接种量为0.5 g/L,转速转换时间为36 h.在此条件下由250 g/L木糖摇瓶发酵252 h得到207.65 g/L木糖醇,转化率为83.1%,达到理论值的91.8%.本研究表明酵母菌SB18在高浓度木糖醇的工业生产中具有很大的应用潜力. |
| 关 键 词： | 木糖醇生产 两级法发酵 热带假丝酵母 木糖还原酶 木糖醇脱氢酶 |
Isolation and Identification of a High-yield Xylitol-producing Yeast Strain and Its Two-stage Fermentation Performances |
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| ZHANG Jin-ming,GENG An-li,YAO Chuan-yi,LU Ying-hua,LI Qing-biao.Isolation and Identification of a High-yield Xylitol-producing Yeast Strain and Its Two-stage Fermentation Performances[J].Journal of Xiamen University(Natural Science),2011,50(3):624-630. | |
| Authors: | ZHANG Jin-ming GENG An-li YAO Chuan-yi LU Ying-hua LI Qing-biao |
| Institution: | ZHANG Jin-ming1,GENG An-li2,YAO Chuan-yi1,LU Ying-hua1,3,LI Qing-biao1,3(1.College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China,2.School of Life Sciences and Chemical Technology,Ngee Ann Polytechnic,Singapore 599489,Singapore,3.The Key Laboratory for Chemical Biology of Fujian Province,China) |
| Abstract: | Xylose-utilizing yeasts isolated from soil samples in Singapore were screened for xylitol production in high xylose concentration.After several round of screening,10 strains were selected for their high tolerance to 300 g/L xylose concentration,which can convert xylose into xylitol with a yield greater than 64.0%.Strain SB18 exhibited the maximal xylitol yield of 68.5% from 150 g/L xylose,and then was identified as Candida tropicalis by 18S rDNA sequencing information.Enzyme assay results indicate that in s... |
| Keywords: | xylitol production two-stage fermentation Candida tropicalis xylose reductase xylitol dehydrogenase |
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