| 黔产紫陀螺菌深层发酵条件的优化及成分分析 |
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| 引用本文： | 宋锡全,王素英,张胜博,周碧珍. 黔产紫陀螺菌深层发酵条件的优化及成分分析[J]. 贵州师范大学学报(自然科学版), 2014, 32(5): 1-4 |
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| 作者姓名： | 宋锡全 王素英 张胜博 周碧珍 |
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| 作者单位： | 贵州师范大学生命科学学院,贵州贵阳,550001 |
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| 基金项目： | 贵州省科学技术基金资助项目[黔科合J字LKS |
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| 摘 要： | 采用摇瓶培养法对紫陀螺菌(Gomphus purpuraceus)适宜的发酵碳源和氮源、发酵条件进行了分析研究,结果表明:最适宜的碳源、氮源分别是葡萄糖和牛肉膏;最优发酵条件的初始pH5.4~6.2,振荡速度145r/min,培养温度26℃,100mL三角瓶装液量50mL。此外,还测定了紫陀螺菌深层培养菌丝体中蛋白质成分中的氨基酸种类。
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| 关 键 词： | 紫陀螺菌 深层发酵 菌丝体 优化 成分分析 |
Study on submerged fermentation and analysis of Gomphus purpuraceus grow in GuiZhou |
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| SONG Xiquan,WANG Suying,ZHANG Shengbo,ZHOU Bizheng. Study on submerged fermentation and analysis of Gomphus purpuraceus grow in GuiZhou[J]. Journal of Guizhou Normal University(Natural Sciences), 2014, 32(5): 1-4 |
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| Authors: | SONG Xiquan WANG Suying ZHANG Shengbo ZHOU Bizheng |
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| Affiliation: | SONG Xiquan;WANG Suying;ZHANG Shengbo;ZHOU Bizheng;School of Biology,Guizhou Normal University; |
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| Abstract: | The authors explore that suitable fermentative conditions affecting the mycelia growth of Gomphus purpuraceus.Experimental results indicated that the optimum composition of a fermentative medium is as following：the most suitable nitrogen source and carbon source are glucose and beef extract ;The highest yield of mycelia was obtained on the conditions that the shaker rotation speed is at 145r/min,p H 5.4 ～ 6.2,the temperature is at 26℃,the optimal culture media capacity is 50mL in 100mLflask.The protein compositions and the kind of amino acids in the submerged culture for mycelia of Gomphus purpuraceus were also detected. |
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| Keywords: | Gomphus purpuraceus submerged fermentation mycelia optimum components analysis |
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