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黔产紫陀螺菌深层发酵条件的优化及成分分析
引用本文:宋锡全,王素英,张胜博,周碧珍. 黔产紫陀螺菌深层发酵条件的优化及成分分析[J]. 贵州师范大学学报(自然科学版), 2014, 32(5): 1-4
作者姓名:宋锡全  王素英  张胜博  周碧珍
作者单位:贵州师范大学生命科学学院,贵州贵阳,550001
基金项目:贵州省科学技术基金资助项目[黔科合J字LKS
摘    要:采用摇瓶培养法对紫陀螺菌(Gomphus purpuraceus)适宜的发酵碳源和氮源、发酵条件进行了分析研究,结果表明:最适宜的碳源、氮源分别是葡萄糖和牛肉膏;最优发酵条件的初始pH5.4~6.2,振荡速度145r/min,培养温度26℃,100mL三角瓶装液量50mL。此外,还测定了紫陀螺菌深层培养菌丝体中蛋白质成分中的氨基酸种类。

关 键 词:紫陀螺菌  深层发酵  菌丝体  优化  成分分析

Study on submerged fermentation and analysis of Gomphus purpuraceus grow in GuiZhou
SONG Xiquan,WANG Suying,ZHANG Shengbo,ZHOU Bizheng. Study on submerged fermentation and analysis of Gomphus purpuraceus grow in GuiZhou[J]. Journal of Guizhou Normal University(Natural Sciences), 2014, 32(5): 1-4
Authors:SONG Xiquan  WANG Suying  ZHANG Shengbo  ZHOU Bizheng
Affiliation:SONG Xiquan;WANG Suying;ZHANG Shengbo;ZHOU Bizheng;School of Biology,Guizhou Normal University;
Abstract:The authors explore that suitable fermentative conditions affecting the mycelia growth of Gomphus purpuraceus.Experimental results indicated that the optimum composition of a fermentative medium is as following:the most suitable nitrogen source and carbon source are glucose and beef extract ;The highest yield of mycelia was obtained on the conditions that the shaker rotation speed is at 145r/min,p H 5.4 ~ 6.2,the temperature is at 26℃,the optimal culture media capacity is 50mL in 100mLflask.The protein compositions and the kind of amino acids in the submerged culture for mycelia of Gomphus purpuraceus were also detected.
Keywords:Gomphus purpuraceus  submerged fermentation  mycelia  optimum  components analysis
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