| 西瓜NPR1抗病基因的遗传转化 |
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| 引用本文： | 秦新民,张丽珍,李文兰,阳晶,秦峰.西瓜NPR1抗病基因的遗传转化[J].广西师范大学学报(自然科学版),2006,24(2):81-85. |
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| 作者姓名： | 秦新民 张丽珍 李文兰 阳晶 秦峰 |
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| 作者单位： | 广西师范大学,生命科学学院,广西,桂林,541004 |
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| 摘 要： | 比较了不同预培养时间、侵染时间、共培养时间等因素对农杆菌转化西瓜的影响。西瓜遗传转化的适宜条件:3 d苗龄的子叶,预培养1 d,农杆菌侵染10~15 m in,共培养3 d;不定芽与根的潮霉素(Hyg)筛选浓度分别为20 m g.L-1和6 m g.L-1。对20株西瓜转化苗进行DNA提取,PCR检测,其中5株呈阳性,初步证明目的基因已经整合到西瓜基因组中。
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| 关 键 词： | 植物学 西瓜 子叶 农杆菌介导法 遗传转化 |
| 文章编号： | 1001-6600（2006）02-0081-05 |
| 收稿时间： | 2005-11-19 |
| 修稿时间： | 2005年11月19 |
Genetic Transformation of Watermelon with Disease Resistance NPR1 Gene |
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| QIN Xin-min,ZHANG Li-zhen,LI Wen-lan,YANG Jing,QIN Feng.Genetic Transformation of Watermelon with Disease Resistance NPR1 Gene[J].Journal of Guangxi Normal University(Natural Science Edition),2006,24(2):81-85. |
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| Authors: | QIN Xin-min ZHANG Li-zhen LI Wen-lan YANG Jing QIN Feng |
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| Abstract: | The Agrobacterium mediated transformation of Citrullus vulgaris was studied.Effects of different time interval of preculture,bacterial infection and co-cultivation on the transgenic efficiency were investigated.The results showed that preculture three days cotyledon for 1 day was in the best adptive condition,bacterial infection was 10~15 min,and co-cultivation for three days.The adventitious bud and root of watermelon were sensitive to hygromycin,20 mg/L and 6 mg/L was the suitable screening concentration.Transforming plantlets were detected by PCR,five transformants had got from 20 plantlets.The results indicated primarily that the target gene was inserted into watermelon genome. |
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| Keywords: | batony Citrullus vulgaris cotyledon Agrobacterium-mediated method genetic transformation |
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