裸小鼠在普通环境中使用IVC笼具生产繁殖的初探

目的探讨BALB/C-nu裸小鼠在普通环境中使用IVC笼具生产繁殖的效果。方法引进3对BALB/C-nu种鼠按1♂nu/+×1♀nu/+交配,再从仔代中建立血缘扩大群,按1♂nu/nu×3♀nu/+;1♂nu/nu×1♀nu/nu两种繁殖方法进行生产繁殖。结果种鼠生产10胎平均产仔8.6±1.02(裸鼠1.8±0.63)只;血缘扩大群1♂nu/nu×3♀nu/+生产42胎,平均产仔6.92±1.47(裸鼠3.52±1.29)只;1♂nu/nu×1♀nu/nu生产12胎,平均产仔4.25±1.36全裸鼠。经检测符合SPF级。结论在普通环境中应用IVC笼具,只要注意室内卫生消毒,在净化工作台上无菌操作能够繁殖合格的裸小鼠。

Studies on Reproductive Capacity of Nude Mice in IVC Cages at Conventional Facility

Objective To observe reproductive capacity of BALB/C-nu in IVC cages at conventional facility.Methods Three pairs of BALB/C-nu stocks(the ratio of female to male was one to one.) were introduced and the BALB/C-nu stocks were mated in pairs,and the expanding population of blood relationship from offspring was reproduced(the ratio of female to male was three to one,or one to one) in IVC cages at conventional facility.Results The average number born from ten births in BALB/C-nu stocks was 8.6±1.02(nude mice: 1.82±0.63),the average number born from 42 births in the expanding population of blood relationship(the ratio of female to male being three to one) was 6.92±1.47(nude mice: 3.52±1.29),and the average number of nude mice born from 12 births in the expanding population of blood relationship(the ratio of female to male being one to one) was 4.25±1.36.The quality of the nude mice was in keeping with SPF standards.Conclusion The certified nude mice could be reproduced in IVC cages at conventional facility if only the rigorous indoor disinfection and sterile working on purification working stable were carried out.