| ACA的间接免疫荧光法显示多种动、植物细胞着丝点 |
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| 引用本文： | 潘维林,王永潮,彭安,汪堃仁,范勰.ACA的间接免疫荧光法显示多种动、植物细胞着丝点[J].北京师范大学学报(自然科学版),1991,27(4):466-470. |
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| 作者姓名： | 潘维林 王永潮 彭安 汪堃仁 范勰 |
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| 作者单位： | 北京师范大学生物系，北京师范大学生物系，北京师范大学生物系，北京师范大学生物系，北京协和医院皮肤科 100875 北京市新外大街，100875 北京市新外大街，100875 北京市新外大街，100875 北京市新外大街，100750 北京市东单3条 |
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| 摘 要： | 用硬化性肌膜炎病人ACA血清的间接免疫荧光法显示了多种动、植物细胞的着丝点,其中包括Hep-2,文昌鱼、水螅、螽蜥、蚯蚓、草履虫、洋葱、大蒜,吊兰、蚕豆和葫芦藓等。表明在进化不同阶段的动、植物细胞均有同源的、高度保守性的着丝点蛋白。
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| 关 键 词： | 抗着丝点抗体 间接免疫荧光法 植物细胞 着丝点 |
EXHIBITION OF KINETOCHORE/CENTROMERE IN A VARIETY OF ANIMAL AND PLANT CELLS BY ACA INDIRECT IMMUNOFLUORESCENT METHOD |
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| Pan weilin Wang Yongchao Peng An Wang Kunren Yuan Xie.EXHIBITION OF KINETOCHORE/CENTROMERE IN A VARIETY OF ANIMAL AND PLANT CELLS BY ACA INDIRECT IMMUNOFLUORESCENT METHOD[J].Journal of Beijing Normal University(Natural Science),1991,27(4):466-470. |
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| Authors: | Pan weilin Wang Yongchao Peng An Wang Kunren Yuan Xie |
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| Institution: | Pan weilin~1) Wang Yongchao~1) Peng An~1) Wang Kunren~1) Yuan Xie~2) 1) Department of Biology,Beijing Normal University,100875,Beijing,PRC, 2) Department of Dermatology,Peking Union Medical College,100750,Beijing,PRC |
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| Abstract: | This work exhibits the existence of the kinetochore/centromere in a variety of animal and plant cells by indirect immunofluorescent method, using ACA sera from patients with sclerotic fasciitis. Many species such as Hep-2, Branchiostoma belcheri tsingtaoensis, Hydra vulgaris attennata, Pheretima tschiliensis, Monoptorus albus, Paramecium caudatum, Gampsocleosis sp., Vicia faba, Allium cepa, Allium sativum, Chlorophylum capense, Funaria hygrometric Hedw are selected as the materials in the study. The research results indicate that all the animals and plants concerned by us, which are located at different evolutionary period in the biological kingdom, have homologous and highly conserved kinetochore protein. |
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| Keywords: | anti-centromere/kinetochor antiboty(ACA) inidirect immunofluorescence ptant cells kinetochore/centromere |
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