| 抗凝血药物华法灵钠与人血清白蛋白的相互作用研究 |
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| 引用本文： | 宋玉民,张琼,李清萍,缐萍,高琦宽.抗凝血药物华法灵钠与人血清白蛋白的相互作用研究[J].西北师范大学学报,2011,47(1). |
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| 作者姓名： | 宋玉民 张琼 李清萍 缐萍 高琦宽 |
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| 作者单位： | 西北师范大学化学化工学院;甘肃省卫生学校; |
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| 基金项目： | 甘肃省自然科学基金资助项目(0710JRZA108); 甘肃省高分子材料重点实验室资助项目 |
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| 摘 要： | 用荧光光谱、紫外光谱和圆二色谱法研究了抗凝血药物华发灵钠与人血清白蛋白(HSA)的相互作用.初步确定华法灵钠对人血清白蛋白的荧光产生猝灭现象,猝灭方式为静态,并计算了二者形成化合物的结合常数K和结合位点数n.根据不同温度下的热力学函数,确定了华发灵钠与人血清白蛋白的相互作用力类型为氢键和范德华力.发现华法灵钠的存在明显改变人血清白蛋白的构象,并讨论了华发灵钠使人血清白蛋白构象发生变化的可能原因.
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| 关 键 词： | 华法灵钠 人血清白蛋白 荧光光谱 紫外吸收光谱 圆二色谱 |
Studies on interaction between the anticoagulants warfarin and human serum albumin |
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| SONG Yu-min,ZHANG Qiong,LI Qing-ping,XIAN Ping,GAO Qi-kuan.Studies on interaction between the anticoagulants warfarin and human serum albumin[J].Journal of Northwest Normal University Natural Science (Bimonthly),2011,47(1). |
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| Authors: | SONG Yu-min ZHANG Qiong LI Qing-ping XIAN Ping GAO Qi-kuan |
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| Institution: | SONG Yu-min1,ZHANG Qiong1,LI Qing-ping1,XIAN Ping1,GAO Qi-kuan2(1.College of Chemistry and Chemical Engineering,Northwest Normal University,Lanzhou 730070,Gansu,China,2.Medical School of Gansu Province,Lanzhou 730000,China) |
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| Abstract: | Fluorescence spectroscopy,UV spectra and circular dichroism are applied to study interaction between human serum albumin(HSA)and anticoagulants warfarin at different temperatures.The quenching mechanism of fluorescence of HSA by warfarin is found to be a static quenching procedure and the binding constant K and binding sites n are calculated.According to the thermodynamic functions at different temperatures,the binding type of warfarin to HSA is hydrongen bond and Van der Waals force.The research results sh... |
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| Keywords: | warfarin human serum albumin fluorescence UV spectra circular dichroism |
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