长春花愈伤组织培养过氧化物酶同工酶等电点聚焦研究

Riicker,W.等(1971)进行了组织培养过氧化物酶等电点聚焦研究,此法反应灵敏,重复性好.我们用红花长春花叶为外植体,进行脱分化培养,诱导产生愈伤组织,并结合用不同激素、r射线等因素处理.同时,在不同培养时间取样,用聚丙烯酰胺圆盘电泳,进行过氧化物酶同工酶等电点聚焦研究,发现在各种因素作用下,过氧化物酶同工酶均有不同的变化,用等电点分离同工酶是植物发育、细胞分化的灵敏指标,为比较研究提供了重要手段.

Isoelectric Patterns of Peroxidase Isozymes from Vinca rosea Tissue Cultures

Peroxidases from Vinca rosea tissue cultures have been separated by polyacrylamide gel electrophoresis and isoelectric focusing into 6-13 isozymes. The isozyme pattern of callus tissues and those of plants at different development stages have been compared. The callus was obviously responsive to exogenous hormones.The isozyme pattern of the calluses cultured with different exogenous hormones was significantly changed. The subcultural and original callus had basically similar isozyme patterns when they were cultured under the same hormones condition.~(60)Co irradiation of callus with dose of 25210 rad did not inhibit cellular growth and produced a new isozyme band.At different developmental stages of the plants, the isozyme patterns of their roots, stems and leaves were not similar, the healthy seedling's have changed much than the young seedling's and those of the old seedlings has three new isozyme bands at pI8-9.5