大肠杆菌质粒DNA不同提取方法的比较

实验采用简化的碱法少量提取法、简便快速的煮沸法及试剂盒法提取质粒 p CB30 2 -3.并通过一对特异引物对质粒 DNA进行 PCR(聚合酶链式反应 )扩增 ,分别对各种方法所提取的质粒 DNA及其 PCR扩增产物进行琼脂糖凝胶电泳分析 ,结果表明碱法少量提取法和试剂盒法所提取的质粒 DNA纯度和产量高 ,且重复性好 ,达到了分子生物学实验要求 .其中简化的碱法少量提取法具有结果稳定和经济的特点 ,非常适合大多数实验室使用 .

The Comparison of Different Extraction Methods of E. Coli Plasmid DNA

In this experimentol study,a-little-amount-extraction-in-base method,kit method,and simple and quick boil method are used to extract plasmid pCB302-3.Then the plasmid DNAs obtained were amplified by polymerase chain reaction(PCR)with the help of a pair of specific primers.Agar sugar gel electrophoresis was used to analyze the respective plasmid DNAs and the amplified ones.Good yields with high purity and good repetition were found to have been obtained in the first two methods,coming up to the requirements for molecular biology experimentation,especially in the first method characterized by stable and economical results.It is therefore concluded that a-little-amount-extraction-in-base method is very suitable to be used by most laboratories.