橘皮黄酮提取的响应面优化及抗氧化活性研究

以黄酮得率为指标,采用Box-Behnken中心组合实验和响应面分析法,研究了料液比、提取温度和提取时间对橘皮中黄酮类化合物提取的影响;用AB-8型大孔吸附树脂为色谱柱填充料,对橘皮黄酮提取物进行了纯化;以橘皮黄酮对1,1-二苯基-2-三硝基苯肼（DPPH）自由基、羟基自由基的清除率为指标,研究了橘皮黄酮的体外抗氧化活性.结果表明：橘皮黄酮的适宜提取工艺是以水为提取溶剂、料液比为1∶38（g/mL）、于99℃浸提2 h,该条件下橘皮黄酮的最大得率为11.028 mg/g;AB-8型大孔吸附树脂对橘皮黄酮类化合物的纯化效果明显,纯化后橘皮黄酮的纯度提高了383.312%;对DPPH自由基、羟基自由基清除率的半抑制浓度（IC50）分别为0.019和0.557 mg/mL,纯化后橘皮黄酮对DPPH和羟基自由基的清除能力分别提高了96.185%和65.122%,表明橘皮黄酮是一种良好的天然抗氧化剂.

The optimical extraction conditions of flavonoids from orange peel by response surface analysis and their antioxidative effects

Box-Behnken central composite test and response surface methodology was used to optimize the extraction conditions of flavonoids from orange peel and AB-8 macroreticular adsorptive resin was used to refine the flavonoids.The antioxidation activity of the product was evaluated.The results showed that the optimum extracting solvent for flavonoids from orange peels was water,and solid to liquid ratio of 1 ∶ 38(g/mL) for an extraction duration of 2 h at 99 ℃.Under these conditions,the yield of flavonoids was 11.028 mg/g;AB-8 macroreticular adsorptive resin was showed to be a perfect adsorbent to refine the extracted flavonoids;It was proved that flavonoids from orange peel,especially the purified product,was a good natural antioxidant.