| 加替沙星自组装环荧光显微成像技术应用于内蒙古品牌乳、胶囊、尿液中加替沙星的检测 |
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| 引用本文： | 余燕敏,杨乐,刘颖.加替沙星自组装环荧光显微成像技术应用于内蒙古品牌乳、胶囊、尿液中加替沙星的检测[J].内蒙古大学学报(自然科学版),2010,41(1). |
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| 作者姓名： | 余燕敏 杨乐 刘颖 |
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| 作者单位： | 1. 中央民族大学生命与环境科学学院,北京,100081
2. 中央民族大学生命与环境科学学院,北京,100081;内蒙古师范大学化学与环境科学学院,呼和浩特,010022 |
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| 基金项目： | 国家自然科学基金(20767004);;中央民族大学“985工程”项目(MUC985);;中央民族大学“211工程”项目(MUC021211306);;中央民族大学引进人才启动基金;;内蒙古自治区自然科学基金重点项目(200711020202);;内蒙古自治区高等学校科学研究项目(NJ06108)资助 |
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| 摘 要： | 利用加替沙星自组装环荧光显微成像技术,建立一种灵敏度高、操作简便的加替沙星固相检测方法.在pH 3.60的HAc-NaAc缓冲溶液和聚乙烯醇-124存在下,加替沙星能够在二氯二甲基硅烷(DMCS)处理过的载玻片上形成自组装环(Self-ordered ring,SOR),环直径为2.45 mm,环线宽为38.7μm.当点样体积为0.30μL时,线性范围6.74×10-13~1.83×10-11mol.ring-1,方法的检出限(3σ)为6.74×10-14mol.ring-1(2.2×10-7mol.L-1).应用于内蒙古乳都品牌乳、加替沙星胶囊和健康志愿者服药后尿液等样品中加替沙星的分析检测,加标回收率为96.0%~106.6%,RSD小于3.3%.
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| 关 键 词： | 自组装环荧光显微成像技术 加替沙星 牛奶 尿液 |
Determination of Gatifloxacin in Brand Milks in Inner Mongolia,Capsules and Human Urine Samples by Self-ordered Ring Fluorescent Microscopic Technique |
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| YU Yan-min,YANG Le,LIU Ying.Determination of Gatifloxacin in Brand Milks in Inner Mongolia,Capsules and Human Urine Samples by Self-ordered Ring Fluorescent Microscopic Technique[J].Acta Scientiarum Naturalium Universitatis Neimongol,2010,41(1). |
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| Authors: | YU Yan-min YANG Le LIU Ying |
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| Institution: | 1.College of Life and Environmental Science/a>;MinZu University of China/a>;Beijing 100081/a>;China/a>;2.College of Chemistry and Environmental Science/a>;Inner Mongolia Normal University/a>;Hohhot 010022/a>;China |
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| Abstract: | A highly sensitive and simple solid-state method was introduced for the determination of gatifloxacin based on the capillary effect of solvent on a solid support with fluorescence microscopic technique.In the pH 3.60 HAc-NaAc buffer solution and Poly(vinyl alcohol)-124 (PVA-124), a typical fluorescent SOR of gatifloxacin can be obtained on dimethyl dichlorosilane(DMCS) pretreated hydrophobic glass slide with outer diameter less than 2.45mm and ring belt width less than 38.7μm.Linear response is observed in the range of 6.74×10~(-13)～1.83×10~(-11)mol·ring~(-1)and the detection limit (3σ) reaches 6.74×10~(-14) mol·ring~(-1)(2.2×10~(-7) mol·L~(-1)) when 0.30 μL droplet is spotted.The assay was successfully applied into the determination of gatifloxacin in brand milks of Inner Mongolia,gatifloxacin capsules and human urine samples of healthy volunteers after dosing with the recovery of 96.0%～106.6% and RSD lower than 3.3%. |
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| Keywords: | self-ordered ring fluorescence microscopic technique gatifloxacin milk human urine |
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