光谱法研究小分子与蛋白质间相互作用的进展

小分子与蛋白质之间相互作用会影响蛋白质构象变化,研究小分子与蛋白质相互作用的机理,对阐明小分子在生物体内的吸收、分布、代谢等过程具有重要意义。综述了近年来紫外光谱法、荧光光谱法、傅里叶变换红外光谱法和圆二色光谱法等技术在该领域的研究进展,分析了这些方法的特点和应用,展望了外源性小分子与蛋白质之间相互作用的发展前景。

Progress of Study on Interaction between Small Molecules and Proteins by Spectroscopy

The interaction between small molecules and proteins can influence conformation of protein. The research results of binding mechanism play an important role revealing the absorption, distribution, metabolism of exogenous small molecules in vivo. This paper focuses on the application of spectroscopic techniques such as UV-vis spectrophotometry, fluorescent spectrometry, fourier transform infrared spectrometry and circular dichroism in this field. The characteristics and application of these methods are summarized. And the prospects for the development of binding mechanism between small molecules and protein are also presented.