响应面法优化斜生栅藻处理高氨氮废水的光照条件

为了减少废水中氨氮对环境的危害,降低培养微藻的成本,利用废水培养斜生栅藻,采用三因素三水平的Box-Behnken设计,采用响应面法研究光波长、光照强度及光照时间对斜生栅藻干重和氨氮去除率的影响,建立回归模型,优化反应条件,为后续研究提供依据。结果表明,最优反应条件为:蓝光照射,光照强度为3 500 lux,光照时间为12 h。在此条件下,测得的藻类干重为(0.63±0.02)g·L~(-1),氨氮的去除率为92.08%±0.20%,与预测值相符。3个因素对藻类干重的影响程度大小依次为光波长光照时间光照强度,3个因素对氨氮去除率的影响程度依次为:光照强度光波长光照时间。

Light Condition Optimization for Removal of High Ammonia Nitrogen in Wastewater Based on Scenedesmus obliquus Using Response Surface Methodology

In order to reduce the environmental hazards of ammonia nitrogen in wastewater and decrease the cost of cultivation of microalgae, Scenedesmus obliquus was used for treatment of ammonia nitrogen to investigate the effects of different light wavelength, light intensity and light cycle on the biomass production rate of Scenedesmus obliquus and removal rate of ammonia nitrogen. Based on response surface methodology, regression model was established to optimize the reaction conditions to provide basic information for subsequent research and development. The results showed that: the optimal culture conditions were determined blue of light wavelength, 3 500 lux of light intensity and 12 h of light cycle. The maximum dry weight of Scenedesmus obliquus was 0.63±0.02 g.L-1, and the removal rate of ammonia nitrogen reached 92.08±0.20 %, which is consistent with the predicted value by response surface method. The order of the factors for dry weight is as follows: light wavelength, light cycle and light intensity. The order of the factors for ammonia removal rate is as follows: light intensity, light wavelength and light cycle.