内蒙古通辽市砂质土壤中微生物群落结构及其与环境因子的关系

在内蒙古通辽市采集6个土壤样品,研究该地区砂质土壤中微生物群落结构及其与环境因子的关系。应用Illumina Mi Seq高通量测序技术发现,相对丰度大于1%的细菌门有放线菌门、变形菌门、厚壁菌门、芽单胞菌门、绿弯菌门、酸杆菌门、拟杆菌门,相对丰度大于5%的真菌门有子囊菌门、担子菌门。典范对应分析显示影响细菌群落结构的环境因素主要有硝态氮、溶解性有机碳、铵态氮、总氮和含砂量,影响真菌群落结构的环境因素主要有溶解性有机碳、硝态氮、p H、总氮和含砂量。方差分解分析表明硝态氮、溶解性有机碳和铵态氮分别解释了20.73%、16.51%和15.59%的细菌群落结构的差异;溶解性有机碳、硝态氮和p H分别解释了22.29%、22.05%和17.76%的真菌群落结构的差异。研究为探索砂质土壤中微生物群落结构及其与环境因子的关系提供了理论依据。

Microbial Community Structure and the Correlation between Environmental Factors and Community Structure in Sandy Soil of TongliaoSinSInnerSMongolia

Six soil samples were collected from Tongliao,Inner Mongolia to study microbial community structure and the correlation between environmental factors and community structure.The results of Illumina MiSeq high-throughput sequencing show that the bacterial phyla of which relative abundance is more than 1% are Actinobacteria, Proteobacteria, Firmicutes, Gemmatimonadetes, Chloroflexi, Acidobacteria and Bacteroidetes; the fungal phyla of which relative abundance is more than 5% are Ascomycota, Basidiomycota. Canonical correspondence analysis shows that the main environmental factors which affect bacterial community structure are nitrate nitrogen, dissolved organic carbon, ammonium nitrogen, total nitrogen and sand content; The main environmental factors that affect the community structure of fungi are dissolved organic carbon and nitrate nitrogen , pH, total nitrogen and sand content. Variation partition analysis shows that nitrate nitrogen, dissolved organic carbon and ammonium nitrogen, explaining 20.73%, 16.51% and 15.59% of the bacterial community differences, while dissolved organic carbon, nitrate nitrogen and pH explain 22.29%,22.05% and 17.76% of the fungal community structure differences. This study provides a theoretical basis for exploring microbial diversity and the relation between community structure and environmental factors in sandy soil.