R—藻红蛋白色基多肽对肿瘤细胞光动力杀伤作用的实验研究

首次用胃酶降解及CM Sephedex C-50柱层析法获得了6种藻红蛋白的色基多肽，并用藻红蛋白及其色基多肽对两种肿瘤细胞作体外激光疗法增敏作用实验，S180小鼠腹水癌细胞株培养后分别用浓度为10，25，50，100ug/mL的藻红蛋白及其色基多肽处理，经波长为488nm的氩离子激光辐照（照射剂量为28．8J/cm2)，用MTT法检测，其细胞的生存率最佳效果分别棕达45％及16％，显示出良好的剂量效应，在激光处理藻红蛋白及其色基多肽对HL60人白血病细胞所做的细胞生长曲线图的比较中可看出，色基多肽的激光增敏作用要优于藻红蛋白多聚体。

The Experimental Researchof R-Phycoerythrin and Its Chromophore Polypeptides in the Photodynamic Therapy of Cancer

It is the first time that six kinds of Chromophore Polypeptides of R-Phycoerythrin (R-PE)were obtained from polysiphona urceolata by pepsin and CM-Sephedex C50 . In order to compare the differetiation between R-PE and its Chromophore Polypeptides, two kinds of tumor cell lines were used for an the experiment of photodynamic therapy (PDT) in vitro. S180 cell line was first treated with different concentrations of R-PE and its Chromophore Polypeptides , and then irradiated with Ar-iron laser ( 488nm, 28.8 J/cm2). The survival rate was measured by MIT method. Moreover, the growth rate of HL60 cells , which was treated with R-PE and its Chromophore Polypeptides. The results show that the PDT effect of Chromophore Polypeptides is better than R-PE.