The collagen substratum influences in vitro hatching and attachment of the mouse blastocyst in a serumless medium |
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| Authors: | R J Wordinger M McGrath |
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| Institution: | (1) Department of Biology, St. Bonaventure University, 14778 St. Bonaventure, New York, USA;(2) Present address: Department of Anatomy, Texas College of Osteopathic Medicine, 76107 Fort Worth, Texas, USA;(3) Present address: Department of Dairy Science, Clemson University, 29631 Clemson, South Carolina, USA |
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| Abstract: | Summary When a serumless medium is used for the in vitro growth and development of post-blastocyst mouse embryos, a collagen substratum causes a delay in the hatching from the zona pellucida. However, the collagen substratum is essential for blastocyst attachment and trophoblast cell outgrowth after hatching has taken place. |
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