Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis |
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Authors: | S. Padilla U. C. Tran M. Jiménez-Hidalgo J. M. López-Martín A. Martín-Montalvo C. F. Clarke P. Navas C. Santos-Ocaña |
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Affiliation: | (1) Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC and Centre for Biomedical Research on Rare Diseases (CIBERER), Carretera de Utrera, km 1, ISCIII, 41013 Sevilla, Spain;(2) Department of Chemistry and Biochemistry, UCLA, Los Angeles, 90095, CA, USA |
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Abstract: | Coenzyme Q is a lipid molecule required for respiration and antioxidant protection. Q biosynthesis in Saccharomyces cerevisiae requires nine proteins (Coq1p–Coq9p). We demonstrate in this study that Q levels are modulated during growth by its conversion from demethoxy-Q (DMQ), a late intermediate. Similar conversion was produced when cells were subjected to oxidative stress conditions. Changes in Q6/DMQ6 ratio were accompanied by changes in COQ7 gene mRNA levels encoding the protein responsible for the DMQ hydroxylation, the penultimate step in Q biosynthesis pathway. Yeast coq null mutant failed to accumulate any Q late biosynthetic intermediate. However, in coq7 mutants the addition of exogenous Q produces the DMQ synthesis. Similar effect was produced by over-expressing ABC1/COQ8. These results support the existence of a biosynthetic complex that allows the DMQ6 accumulation and suggest that Coq7p is a control point for the Q biosynthesis regulation in yeast. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. Received 04 September 2008; received after revision 22 October 2008; accepted 23 October 2008 |
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Keywords: | KeywordHeading" >. Ubiquinone coenzyme Q mitochondria yeast regulation |
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