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| 野大麦组织培养及植株再生的研究 | |
| 引用本文： | 张立营,李喜文,许红力,庞晓斌,李彦舫.野大麦组织培养及植株再生的研究[J].内蒙古民族大学学报(自然科学版),2004,19(3):290-292. |
| 作者姓名： | 张立营 李喜文 许红力 庞晓斌 李彦舫 |
| 作者单位： | 1. 中国人民解放军军需大学,植物基因工程研究中心,吉林,长春,130062 2. 东北师范大学,遗传与细胞研究所,吉林,长春,130024 3. 解放军208医院,吉林,长春,130062 4. 内蒙古民族大学,农学院,内蒙古,通辽,028042 |
| 摘 要： | 以野大麦成熟胚为外植体，接种于MS 2，4-D2．0mg／L KT0．2mg／L NAA0．5mg／L Su3％的培养基中，诱导愈伤组织；然后在培养基MS 2，4-D2．0mg／L KT0．2mg／L NAA1．0mg／L Su3％上进行继代培养，每次继代时间为20天左右；再用MS KT2．0mg／L NAA0．2mg／L Su1．5％进行分化，分化率达80％以上． |
| 关 键 词： | 野大麦 组织培养 愈伤组织 |
| 文章编号： | 1671-0185(2004)03-0290-03 |
| 修稿时间： | 2004年1月25日 |
Research of Tissue Culture and Plantlet Regeneration of Wild Barley |
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| ZHANG Li-ying,LI Xi-wen,XU Hong-li,Pang Xiao-bin,LI Yan-fang.Research of Tissue Culture and Plantlet Regeneration of Wild Barley[J].Journal of Inner Mongolia University for the Nationalities(Natural Sciences),2004,19(3):290-292. | |
| Authors: | ZHANG Li-ying LI Xi-wen XU Hong-li Pang Xiao-bin LI Yan-fang |
| Institution: | ZHANG Li-ying~1,LI Xi-wen~2,XU Hong-li~3,Pang Xiao-bin~4,LI Yan-fang~1 |
| Abstract: | Mature seeds from Wild Barley(Hordeum brevisubulatum(Trin.)Link) are chosen as explants,which are cultured in tubes containing Murashige and skoog inorganic nutrients (MS) supplemented with 2.0 mg/L of 2,4-D and 0.2 mg/L of KT and 0.5 mg/L of NAA to induce callus;then the callus obtained from tissue cultures are subcultured into fresh medium consisting of MS supplemented with 2.0 mg/L of 2,4-D and 0.2 mg/L of KT and 1.0 mg/L of NAA,each subcultured interval is about 20 days; At last, the subcultured callus are for plants with medium consisting of MS supplemented with 2.0 mg/L of KT and 0.5 mg/L of NAA, the differientiation rate is no less than 80%. |
| Keywords: | Wild barley(Hordeum brevisubulatum (Trin )Link) Tissue culture Callus |
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