| 用siRNA抑制胃癌细胞AGS中COX-2表达及对AKT磷酸化的抑制 |
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| 引用本文： | 刘艳丽 闫岩 白文涛 张芳琳 吴兴安 张德新 徐志凯. 用siRNA抑制胃癌细胞AGS中COX-2表达及对AKT磷酸化的抑制[J]. 科学技术与工程, 2006, 6(8): 1055-10571068 |
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| 作者姓名： | 刘艳丽 闫岩 白文涛 张芳琳 吴兴安 张德新 徐志凯 |
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| 作者单位： | 第四军医大学微生物学教研室,西安,710032;西京医院消化内科,西安,710032 |
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| 摘 要： | 构建了可在真核细胞中表达针对COX-2基因的siRNA表达载体，并用胃腺癌细胞系AGS建立了COX-2 siRNA的稳定转染细胞系。用Western-Blot检测稳定转染细胞系中COX-2的表达以及AKT的磷酸化水平。结果表明稳定转染C0X-2 siRNA的细胞中COX-2的表达较转染空载体的细胞明显降低，且发现COX-2表达的降低可抑制AKT—Ser^473和Thr-^308的磷酸化水平。为进-步探索抑制COX-2对胃癌细胞生物学特性的影响，及其在肿瘤治疗中的作用奠定了基础。
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| 关 键 词： | siRNA COX-2 AGS细胞 |
| 文章编号： | 1671-1815(2006)08-1055-04 |
| 收稿时间： | 2006-01-06 |
| 修稿时间： | 2006-01-06 |
Inhibition of AKT Phosphorylation by COX-2 siRNA in AGS Gastric Cancer Cells |
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| LIU Yanli,YAN Yan,BAI Wentao,ZHANG Fanglin,WU Xingan,ZHANG Dexin,XU Zhikai. Inhibition of AKT Phosphorylation by COX-2 siRNA in AGS Gastric Cancer Cells[J]. Science Technology and Engineering, 2006, 6(8): 1055-10571068 |
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| Authors: | LIU Yanli YAN Yan BAI Wentao ZHANG Fanglin WU Xingan ZHANG Dexin XU Zhikai |
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| Abstract: | Eukaryotic small interfering RNAs (siRNA) expression vector specific for Cyclooxygenase-2 (COX-2) was constructed and transfected into gastric adenocarcinoma cell line AGS. The expression of COX-2 and the phosphorylation of AKT were detected by Western-blot. The results showed that the expression of COX-2 was inhibited in COX-2 siRNA stable transfectants compare with empty vector transfectants, and the AKT phosphorylation at Ser473 and Thr308 was also inhibited by COX-2 siRNA. These findings provide new insights into the roles of COX-2 in gastric carcinogenesis and possible targets for chemoprevention. |
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| Keywords: | siRNA COX-2 AGS cell |
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