| 联用色谱和化学计量学方法分析赤芍挥发性成分 |
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| 引用本文： | 李国辉,兰正刚,李晓如.联用色谱和化学计量学方法分析赤芍挥发性成分[J].中南大学学报(自然科学版),2007,38(1):89-92. |
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| 作者姓名： | 李国辉 兰正刚 李晓如 |
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| 作者单位： | 中南大学化学化工学院，中南大学化学化工学院，中南大学化学化工学院 湖南长沙，410083湖南城市学院化学系，湖南益阳，413000，湖南长沙，410083，湖南长沙，410083 |
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| 摘 要： | 采用气相色谱-质谱(GC-MS)检测,通过直观推导式演进特征投影法(HELP)对二维色谱／质谱数据进行解析,得到各组分的纯色谱曲线和质谱,从而实现对赤芍挥发性成分的定性和定量分析。根据色谱保留时间和质谱,在质谱库中进行相似检索,实现对组分的定性分析,然后用归一化法进行定量分析。研究结果表明:从赤芍挥发性成分中共分辨出57个色谱峰,通过质谱库对分辨的纯组分进行检索,鉴定其中36个成分,占挥发性成分总含量的95．21％;赤芍主要挥发成分为水杨醛、正十六碳酸和(Z,Z)-9,12-十八碳二烯酸。
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| 关 键 词： | 赤芍 挥发性成分 HELP法 |
| 文章编号： | 1672-7207（2007）01-0089-04 |
| 修稿时间： | 2006-05-18 |
Determination of volatile chemical components of Radix Paeoniae Rubra by GC-MS and HELP method |
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| LI Guo-hui,LAN Zheng-gang,LI Xiao-ru.Determination of volatile chemical components of Radix Paeoniae Rubra by GC-MS and HELP method[J].Journal of Central South University:Science and Technology,2007,38(1):89-92. |
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| Authors: | LI Guo-hui LAN Zheng-gang LI Xiao-ru |
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| Institution: | 1, School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; 2. Department of Chemistry, Hunan City College, Yiyang 413000, China |
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| Abstract: | The volatile chemical components of radix paeoniae rubra (RPR) were analyzed qualitatively and quantitatively by gas chromatography-mass spectrometry (GC-MS),heuristic evolving latent projections (HELP) method and normalization method.According to retention time of chromatography and mass spectra,similarity searches were conducted in the MS database,and the chemical components of volatile oil in RPR were analyzed qualitatively,and then quantitative calculation was carried out using normalization method.Thirty-six of volatile chemical components of RPR were determined,accounting for 95.21% of total contents of volatile chemical components of RPR.The mail volatile chemical components of RPR are (Z,Z)-9,12-octadecadienoic acid,n-hexadecanoic acid and 2-hydroxy-benzaldehyde. |
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| Keywords: | GC/MS |
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