| 稀有放线菌分离方法的研究 |
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| 引用本文： | 杨宇容,金湘.稀有放线菌分离方法的研究[J].云南大学学报(自然科学版),1997,19(4):403-408,413. |
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| 作者姓名： | 杨宇容 金湘 |
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| 作者单位： | 云南大学省微生物研究所，新疆农科院微生物研究所，青海中科院西北高原生物研究所 |
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| 基金项目： | 国家自然科学基金,云南省国际科技合作基金,云南大学211工程基金 |
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| 摘 要： | 在培养基中加入７种不同抑制剂，不同的碳源或氮源及对土壤样品进行预处理等选择性分离各类放线菌，结果发现对土壤真菌，细菌有明显抑制作用而对放线菌无抑制作用的只有重铬酸钾，认为它是选择性分离放线菌的一种高效，便宜的抑制剂。在培养基中加入的碳源或氮源不同，可分离到不同类型的放线菌，找到了在分离不同类型的放线菌时培养基中较好的碳，氮源组合。
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| 关 键 词： | 放线菌 分离 抑制剂 氮源 碳源 |
A Study on Isolation Methods of Rare Acinomycetes |
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| Yang Yuroug,Xu Lihua,Duan Ruoling,Jiang Chenglin,Jin Xiang,Wang Qilang.A Study on Isolation Methods of Rare Acinomycetes[J].Journal of Yunnan University(Natural Sciences),1997,19(4):403-408,413. |
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| Authors: | Yang Yuroug Xu Lihua Duan Ruoling Jiang Chenglin Jin Xiang Wang Qilang |
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| Institution: | Yang Yuroug 1) Xu Lihua 1) Duan Ruoling 1) Jiang Chenglin 1) Jin Xiang 2) Wang Qilang 3) |
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| Abstract: | The paper reports the results on selective isolation of actinomycetes by adding seven kinds of bacteristatic agent,and various carbon and nitrogen source in the medium. The results presented that potassium dichromate could inhibit obviously growth of fungus and bacteria in soil samples except actinomycetes,it was a high efficient,cheap bacteriostatic for selective isolation of actinomycetes,A suitable ratio of carbon-nitrogin source in the medium was found for selective isolation of various actinomycetes. The selective isolation procedure was established for isolation rare actinomycetes. Medium YIM-2 was recommened for selective isolation of rare actinomycetes. The HV medium was improved. |
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| Keywords: | rare actinomycetes isolation methods bacteristatic carbon and nitrogen source |
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