电刺激对失重导致的废用性骨骼肌萎缩的影响

骨骼肌萎缩是由于去神经支配、损伤、关节固定、卧床休息、败血症、癌症、衰老以及微重力等引起的骨骼肌质量下降.论文采用骨骼肌电刺激(Muscle Electrical Stimulation,MES)模拟不同运动形式探讨延缓尾悬吊(Tail Suspension,TS)导致的斯泼累格·多雷大鼠(Sprague-Dawley Rat,SD Rat)骨骼肌萎缩的效果,同时引入联合训练模式,进一步探讨维持习惯性运动模式与非习惯性运动模式对延缓骨骼肌萎缩的效果,并结合Sigma算法与传统统计方法对实验结果进行分析.实验结果表明:(1)高频电刺激(High Frequency Electrical Stimulation,HFES)干预6周后可以显著提高骨骼肌质量,其中比目鱼肌质量(m_LS)与胫骨前肌质量(m_LT)分别提高21%(P<0.01)和26%(P<0.01),Akt与p70s6k的蛋白激活程度显著提高;低频电刺激(Low Frequency Electrical Stimulation,LFES)干预6周后,比目鱼肌与胫骨前肌的质量有...

The Effect of Electrical Stimulation on Weightlessness-Caused Waste Skeletal Muscular Atrophy

Skeletal muscular atrophy refers to the decrease of skeletal muscle mass caused by denervation, injury, joint fixation, bed rest, septicemia, cancer, aging and microgravity. Skeletal muscle electrical stimulation (MES) was used to simulate different forms of exercise to explore the effect of delaying skeletal muscular atrophy in Sprague-Dawley (SD) rats caused by tail suspension (TS). The combination training mode was introduced to further explore whether the effect of maintaining habitual exercise mode on delaying skeletal muscular atrophy is better than that of non-habitual exercise mode. The sigma algorithm and traditional statistical methods were used to analyze the experimental results. The results showed that after 6 weeks of high frequency electrical stimulation (HFES) intervention, skeletal muscle mass was significantly improved, in which mass of left soleus (m_LS) and left tibialis (m_LT) increased by 21% (P < 0.01) and 26% (P < 0.01) respectively. The protein activation of Akt and p70s6k increased significantly. After 6 weeks of low frequency electrical stimulation (LFES) intervention, the mass of soleus and tibia-lis tended to increase, but there was no significant difference compared with group Z. After 4 weeks of TS treatment, the mass of soleus decreased by 45% (P < 0.01) compared with group Z, indicating that the model was successfully constructed. The degree of decline was significantly improved after normal gravity and microgravity intervention, and the mass of soleus increased by 49% (P < 0.01) compared with group T after normal gravity intervention. The intervention results of combination group showed that compared with group T, the mass of soleus and tibialis in combination group (group HHT) increased by 55% (P < 0.01) and 37% (P < 0.01) respectively, the mass of left gastrocnemius (m_LG) increased by 25% (P < 0.01), and the overall intervention effect was better than that of other groups. In conclusion, the effect of high-frequency electrical stimulation on increasing skeletal muscle mass is better than that of low-frequency electrical stimulation, and the effect of normal gravity intervention is better than that of microgravity intervention, and the habitual exercise intervention mode is better than that of the non-habitual exercise in delaying soleus muscular atrophy caused by weightlessness.