Effects of dietary nickel on detoxification enzyme activities in the midgut of <Emphasis Type="Italic">Spodoptera litura</Emphasis> Fabricius larvae |
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Authors: | HongXia Sun Qiang Zhou WenCheng Tang YingHua Shu GuRen Zhang |
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Institution: | (1) State key laboratory for Biological Control & Institute of Entomology, Sun Yat-sen University, Guangzhou, 510275, China |
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Abstract: | Nickel accumulated in midugt of Spodoptera litura Fabricius could induce the expression of metallothionein, one of the most important detoxification proteins in organisms.
In the present study, the effects of dietary nickel on the activities of detoxification enzymes, such as carboxylesterase
(CarE) and glutathione S-transferase (GST) in the midgut of S. litura larvae have been studied to get an understanding of the detoxification mechanisms of S. litura larvae to excessive nickel. Results showed that CarE activities in the midgut of the 5th instar larvae decreased at lower
levels of nickel (⩽5 mg/kg), while increased with increasing nickel doses at higher levels of nickel (⩾10 mg/kg) exposure
in successive 3 generations. CarE activities of the 6th instar larvae were also characterized as inhibited at low levels of
nickel exposure, and improved at higher levels in the 1st generation. CarE activities of 6th instar larvae in the 2nd and
3rd generations were all lower than that in control. However, GST activities in the midgut of the 5th and 6th instar larvae
all increased with increasing nickel doses (1–20 mg/kg) in diets.
Supported by the National Basic Research Development Program of China (Grant No. 2006CB102001), National Natural Science Foundation
of China (Grant No. 30771458), Doctoral Fundaytion of Ministry of Education of China (Grant No. 20070558029) and Basic Research
Group Programs of Guangdong Province (Grant No. E039254) |
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Keywords: | nickel Spodoptera litura midgut carboxylesterase glutathione S-transferase |
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