基于羧甲基淀粉的止血防粘连屏障的制备及评价

制备了羧甲基淀粉海绵体,使用氯化钙和甘油调节海绵体材料的孔隙率和韧性,并进一步对材料的细胞相容性、体外凝血性能和体内止血性能进行分析。将优选出的羧甲基淀粉海绵体与电纺膜复合得到止血防粘连屏障,并使用大鼠盲肠磨损模型对材料的防粘连效果进行了详细考察。结果表明,相较于单独使用电纺膜,止血防粘连屏障可以大大降低粘连等级、强度和发生率,防粘连效果更佳。这得益于在止血过程中,海绵体能够慢慢发生溶胀形成具有一定黏性的凝胶,既可以对伤口产生压迫有助于止血,又可以促进防粘连层贴附于伤口,有效阻止防粘连层移位。

Preparation and characterization of a hemostatic anti-adhesive barrier based on carboxymethyl starch

A biodegradable hemostatic anti-adhesive barrier has been fabricated,in which an electrospun membrane (EM) was wrapped between carboxymethyl starch (CMS) sponge.In the sponge,CaCl2 and glycerol were used to improve the porosity and flexibility.The anti-adhesive property was evaluated using a rat model involving sidewall defect-bowel abrasion.Compared to the EM control group,the rats treated with the hemostatic anti-adhesive barrier showed significant reductions in post-operative adhesion in terms of level,tenacity and occurrence.The CMS sponge can swell into a sticky gel in the peritoneal cavity,which contributes to the rapid stop in bleeding and ensures that the electrospun membrane becomes tightly adhered to the wound.