| MTT比色法在检测重组人GM—CSF生物活性中的应用 |
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| 引用本文： | 张冬梅,施晓青,朱洁,秦浚川. MTT比色法在检测重组人GM—CSF生物活性中的应用[J]. 南京大学学报(自然科学版), 1997, 33(2): 253-258 |
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| 作者姓名： | 张冬梅 施晓青 朱洁 秦浚川 |
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| 作者单位： | 南京大学生物化学系医药生物技术国家重点实验室 |
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| 摘 要： | 探索了ＭＴＴ比色法的一系列实验条件，并利用ＧＭ－ＣＳＦ依赖细胞株ＴＦ－１，测定了家蚕基因工程生产的重组人ＧＭ－ＣＳＦ（ｇｒａｎｕｌｏｃｙｔｅ－ｍａｃｒｏｐｈａｇｅｃｏｌｏｎｙ－ｓｔｉｍｕｌａｔｉｎｇｆａｃｔｏｒ）的生物活性，并与半固体培养集落刺激法进行了比较。
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| 关 键 词： | MTT，TF－1细胞株，粒细胞巨噬细胞集落刺激因子 |
APPLICATION OF THE MTT COLORIMETRIC METHOD ON THE BIOLOGICAL ACTIVITIES ASSAY OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR(GM-CSF) |
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| Zhang Dongmei Qin Junchuan Shi Xiaoqing Zhu Jie. APPLICATION OF THE MTT COLORIMETRIC METHOD ON THE BIOLOGICAL ACTIVITIES ASSAY OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR(GM-CSF)[J]. Journal of Nanjing University: Nat Sci Ed, 1997, 33(2): 253-258 |
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| Authors: | Zhang Dongmei Qin Junchuan Shi Xiaoqing Zhu Jie |
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| Abstract: | The cleavage of the tetrazolium salt MTT into a blue coloured product (formazan) by the mitochondrial enzyme succinate dehydro genase is potentially very useful for assaying cell survival and proliferation.The conversion takes place only in living cells and the amount of formazan produced is proportional to the number of cells present. We have established the method that is optimal to the biological activities assay of the rhGM CSF using the TF 1 cell line.Meanwhile,the result is compared to that obtained from colony forming methods. |
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| Keywords: | MTT MTT formazan TF 1 GM CSF |
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