| 裂殖壶菌Schizochytrium sp.FJU-512细胞油脂的研究 |
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| 引用本文： | 张娟梅,江贤章,黄建忠. 裂殖壶菌Schizochytrium sp.FJU-512细胞油脂的研究[J]. 福建师范大学学报(自然科学版), 2007, 23(2): 75-80 |
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| 作者姓名： | 张娟梅 江贤章 黄建忠 |
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| 作者单位： | 福建师范大学生命科学学院,福建,福州,350108 |
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| 基金项目： | 国家自然科学基金 , 福建省教育厅科研项目 |
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| 摘 要： | 裂殖壶菌Schizochytrium sp.FJU-512是高产二十二碳六烯酸(DHA)的一株海洋真菌,其细胞内油脂占细胞干质量的18.3%.细胞油脂的总脂肪酸组成为45.7%DHA,12.5%DPA和41.8%棕榈酸.总油脂由26.4%极性脂和73.6%中性脂组成.薄层色谱、高效液相色谱及质谱分析表明该菌株还能合成强抗氧化剂虾青素.采用均匀设计获得高产DHA的最优培养基(g/L):葡萄糖109.7,蛋白胨19.2,酵母膏20.0,海水晶10.4,DHA产量高达2.10 g/L.
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| 关 键 词： | DHA 裂殖壶菌 气相色谱 薄层色谱 虾青素 |
| 文章编号： | 1000-5277(2007)02-0075-06 |
| 修稿时间： | 2006-08-28 |
Studies on Cell Oil Produced by Schizochytrium sp.FJU-512 |
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| ZHANG Juan-mei,JIANG Xian-zhang,HUANG Jian-zhong. Studies on Cell Oil Produced by Schizochytrium sp.FJU-512[J]. Journal of Fujian Teachers University(Natural Science), 2007, 23(2): 75-80 |
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| Authors: | ZHANG Juan-mei JIANG Xian-zhang HUANG Jian-zhong |
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| Abstract: | Schizochytrium sp.FJU-512 was a marine fungus producing a large amount of docosahexaenoic acid(DHA).The content of the total lipid in its dry cell was up to 18.3%.The profile of fatty acids in the total lipid consisted of 45.7% DHA,12.5% docosapentaenoic acid(DPA) and 41.8% palmitic acid.The total lipid contained 26.4% polar lipid and 73.6% neutral lipid.HPLC and MS analysis showed the strain could also produce astaxanthin,which could protect the cell oil against oxidization.The optimum medium for DHA production were as following: glucose(110.0 g/L) as carbon source,peptone(5.0 g/L) together with yeast extract(18.0 g/L) as nitrogen source;salt 12.5 g/L.The highest DHA-production reached 2.1 g/L at the optimize medium. |
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| Keywords: | Docosahexaenoic acid Schizochytrium gas-chromatography thin layer chromatography astaxanthin |
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