奶犊牛直肠主要正常菌群的检测及其定植规律的研究

采用稀释滴种法，对奶犊牛直肠内容物中８种主要正常菌群（双歧杆菌、乳杆菌、真杆菌、类杆菌、大肠杆菌、消化球菌、梭菌、肠球菌）进行定性和定量分析，将所得结果进行统计学处理，得到奶犊牛直肠８种主要正常菌群的对数均值．犊牛出生６ｈ后可检测到大肠杆菌、类杆菌和梭菌；１２ｈ后，８种菌群全部检测出来，并以大肠杆菌、梭菌为优势菌。随着厌氧菌大量增殖，需氧菌和兼性厌氧菌逐渐下降，１周后形成以乳杆菌、双歧杆菌为优势菌群的微生态区系．８种细菌的数量随饲草采食量增加呈下降趋势．断奶后１个月完成定植，此时直肠的优势菌是双歧杆菌、真杆菌，为７～８ｌｏｇＣＦＵ／ｇ，然后依次为乳杆菌、梭菌７ｌｏｇＣＦＵ／ｇ，类杆菌、大肠杆菌、消化球菌６ｌｏｇＣＦＵ／ｇ，肠球菌５ｌｏｇＣＦＵ／ｇ．

Study on Fixing Planting of the Main Normal Microbial Flora in Calf Rectum

It is carried out with the method of diluting dripping and planting to make qualitive and quantitive analysis on eight kinds of bacteria f rom contents of rectum in milk calf.The results are treated in Statistics and th e eight kinds of main normal Microbial frora logarithmic average value are obtai ned.The results show that Escherichia coli,Bacteroides and Clostridium can b e examined 6?h after birth.All the eight kinds of main normal Microbial frora, Escherichia coli and Clostridium as the dominant Microbial flora,can be examined 12?h after birth.The aerobic bacteria and facultative anaerobic bacteria are de creasing gradually with the multiplication of anaerobic bacteria, Lactobacillus and Bifidobacterium are the dominant Microbial frora on week after birth. The number of the eight kinds of main normal Microbial flora is decreasing with the increment of forage after weaning,it is about thirty days for the main Micro bial flora to fix planting in rectum of milk calf.Meanwhile,the eight main norma l Microbial flora logarithmic average value respectively are as follows: Bifido bacterium and Eubacterium are 7 8?log?CFU/g; Lactobacillus and Clo stridium are 7?log?CFU/g; Bacteroides,Escherichia coli and Peptococcus are 6?log?CFU/g; Enterococcus is 5?log?CFU/g.