Hybrid plant regeneration from interfamilial somatic hybridization between grapevine (vitia vinifera L.) and Red Thorowax (Bupleurum scorzonerifolium willd) |
| |
Authors: | Xiquan Song Guangmin Xia Aifen Zhou Xuezhen Bao Huimin Chen |
| |
Institution: | (1) School of Life Sciences, Shandong University, 250100 Jinan, China;(2) Present address: Biotechnology Department of Guizhou Teachers University, 550001 Guiyang, China |
| |
Abstract: | The protoplasts of Red Thorowax (Bupleurum scorzonerifolium) irradiated by ultraviolet light (UV) at an intensity of 260 μW/cm2 for 0, 1,2 and 3 min respectively were fused with that of grapevine (Vitia vinifera). The regenerated 19 clones, every one derived from a single fused cell, were identified as hybrids by phenotype, isozyme,
chromosome and 5S rDNA spacer region analysis. The results reveal that all of them are somatic hybrids. 11 hybrid calli including
asymmetric and symmetric products regenerated somatic embryos and young leaves after 5 months of culture, of which 4 hybrid
cell lines derived from asymmetric fusion regenerated plants with roots after 8–10 months of culture. Inspection of chromosome
showed that regeneration of whole plant was related to the decrease of chromosome number. Identification of 5S rDNA spacer
region of the plants confirmed that they were interfamilial hybrid plants. |
| |
Keywords: | Grapevine (Vitia vinifera) Red Thorowax (Bupleurum scorzonerifolium) ultraviolet light (UV) somatic hybridization interfamilial hybrid plants |
本文献已被 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |