Genetic analysis on 3′- terminal flanking region of uncoupling protein 3 in different pig breeds |
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Authors: | Meiying Fang Xingbo Zhao Ning Li Changxin Wu |
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Institution: | (1)College of Animal Science and Technology, China Agricultural University ,Beijing 100094 ,China;
(2)State Key Laboratory for Agrobiotechnology, China Agricultural University ,Beijing 100094 ,China |
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Abstract: | The 3′-terminal flanking region of porcine uncoupling protein 3 (UCP3) was cloned, the sequence data revealed 15 nucleotide substitutions among Landrace and three Chinese native pig breeds named Neijiang, Minpig and Erhualian. The continuous 9 polymorphic sites were checked by PCR-RFLP, the results indicatedthat Erhualian had extraordinary gene frequency, presented most significant difference by χ2 test compared with Landrace, Largewhite, Neijiang and Minpig respectively, significant level compared with Meishan; and Meishan also had significant difference compared with Landrace and Minpig respectively. These results canbe concluded that Taihu pigs have special genetic characteristics among pig breeds. |
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Keywords: | pig uncoupling protein gene clone PCR-RFLP |
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