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| 大豆样品中DNA的提取与外源基因CaMV35S的PCR检测 | |
| 引用本文： | 尚智美,高宏伟,王学亮,孙伟.大豆样品中DNA的提取与外源基因CaMV35S的PCR检测[J].菏泽学院学报,2006,28(2):73-75. |
| 作者姓名： | 尚智美 高宏伟 王学亮 孙伟 |
| 作者单位： | 1. 青岛科技大学化学与分子工程学院,山东,青岛,266042 2. 青岛出入境检验检疫局,山东,青岛,266002 3. 菏泽学院化学系,山东,菏泽,274015 |
| 基金项目： | 山东省青岛市自然科学基金 |
| 摘 要： | 采用试剂盒从大豆样品中成功提取出DNA,对花椰菜花叶病毒(Cauliflower mosaic virus,CaMV)35S启动子进行了PCR扩增,并对扩增产物进行了电泳检测,建立了提取大豆DNA与CaMV35S启动子PCR检测的方法,对实验操作中的问题进行了探讨. |
| 关 键 词： | 转基因大豆 PCR CaMV35S DNA |
| 文章编号： | 1673-2103（2006）02-0073-03 |
| 收稿时间： | 2005-11-15 |
| 修稿时间： | 2005年11月15 |
Extraction of Bean DNA and Detection of Transgenic CaMV35S by PCR Method |
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| SHANG Zhi-mei,GAO Hong-wei,WANG Xue-liang,SUN Wei.Extraction of Bean DNA and Detection of Transgenic CaMV35S by PCR Method[J].Journal of Heze University,2006,28(2):73-75. | |
| Authors: | SHANG Zhi-mei GAO Hong-wei WANG Xue-liang SUN Wei |
| Institution: | 1. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao Shandong 266042, China ; 2. Qingdao Entry - Exit Inspection and Quarantine Bureau, Qingdao Shandong 266002, China; 3.Chemistry Department, Heze Univevsity, Heze Shandong 274015, China |
| Abstract: | The DNA was extracted from bean samples and the transgenic elements of cauliflower mosaic virus(CaMV) 35S promoter was amplified by polymerase chain reaction(PCR) method.The PCR products were detected by electrophoresis system.The method was stable and sensitive for the CaMV35S detection and the common problems in PCR procedure were discussed. |
| Keywords: | genetically modified bean PCR CaMV35S DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |