Spectral changes resulting from the interaction of some N-alkyl nitrosamines and rat liver microsomes

Summary Dimethyl (DMN) and diethyl nitrosamine (DEN) do not give characteristic spectral changes upon interaction with rat liver microsomes, while dipropyl (DPN) and dibutyl (DBN) nitrosamine cause type I spectral changes. The spectral binding constant is 100 mM for DPN and 1.17 mM for DBN. The maximal spectral change is 3.2×10⁶ and 1.0×10⁶ absorbance units per milligram protein for DPN and DBN respectively.Acknowledgment. This work was supported by Grants AM 13195-07 from the National Institute of Health (USA) and from the Consejo Nacional de Investigaciones Cientificas y Técnicas (Argentina).