西昌某牛场疑似牛支原体肺炎RT-PCR方法的诊断

应用RT-PCR方法对西昌市某牛场采集的2份疑似牛支原体肺炎鼻腔拭子进行检测,结果显示,两份样品均扩增出预期大小(300bp)的DNA片段。将其纯化回收、克隆、测序,结果表明,两份样品均与GenBank中牛支原体(Mycoplasma bovis)16SrRNA序列(GenBank登录号:AF332754)同源性为99%;牛支原体可能是导致牛发生肺炎的原因之一。

Using RT-PCR to Diagnose the Suspected Mycoplasma Bovis Pneumonia of Cows on a Farm in Xichang

By using RT-PCR to test two suspected mycoplasma bovis pneumonia swab samples collected from the nose of a cow on a farm in Xichang,the result shows that both two samples enlarge and increase a DNA fragment with participated size(300bp).By purifying and recovering it,cloning it and testing its order,the result shows that the homology of two samples with the 16SrRNA series of mycoplasma bovis in GenBank(GenBank passwords: AF332754)is 99%.Mycoplasma bovis is likely to be one of the reasons to cause cow's pneu...